Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Lactose nonfermenters#CC293D
Composition**
| Ingredients | Gms / 1110 ml |
|---|---|
| Peptone from soyabean | 5.000 |
| Sodium chloride | 8.000 |
| Monopotassium phosphate | 1.600 |
| Magnesium chloride, 6H2O | 40.000 |
| Malachite green | 0.040 |
| Final pH (at 25°C) | 5.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.07 grams (the equivalent weight of dehydrated medium per litre) in 1000 ml distilled water. Heat gently if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 115°C for 15 minutes.
Principle And Interpretation
Modified Rappaport Vassiliadis Medium is a selective broth for the enrichment of Salmonella from foodstuffs, environment and clinical specimens. The original formulation described by Rappaport et al (1) with magnesium chloride hexahydrate was modified by Vassiliadis et al (2) by lowering the concentration of malachite green and raising the incubation temperature to 43°C. This medium is recommended as the selective enrichment medium for isolation of Salmonella from food and environmental specimens.
The test specimen is added to Buffered Peptone Water (GM614/M614) and incubated at 35°C for 16 - 20 hours. This pre-enriched peptone water culture is inoculated into Modified Rappaport Vassiliadis Medium and incubated at 42 ± 1°C for 24 - 48 hours and further subcultured on Brilliant Green Agar (M016).
Quality Control
Appearance
Light yellow to light blue coloured granular medium
Colour and Clarity of prepared medium
Blue coloured clear solution without any preciitate
Reaction
Reaction of 3.0% w/v aqueous solution at 25°C. pH: 5.2±0.2
pH
5.00-5.40
Cultural Response
Cultural characteristics observed after an incubation at different temperatures for 24-48 hours, when subcultured on Brilliant Green Agar Base (M016) and then incubated at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Recovery at 37°C |
Recovery at 42 ± 1°C |
Colour of colony |
|---|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | fair | poor | yellowish green |
| Salmonella Paratyphi B ATCC 8759 | 50-100 | good | good | pink white |
| Salmonella Enteritidis ATCC 13076 | 50-100 | luxuriant | luxuriant | pink white |
| Salmonella Typhi ATCC 6539 | 50-100 | fair-good | fair | pink red |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | luxuriant | pink white |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Rappaport F., Konforti N. and Navon B., 1956, J. Clin. Path., 9:261.
- Vassiliadis P. Pateraki E., Papaiconomou N., Papadaicis J. A., Trichopoulos D., 1976, Annales de Microbiologie (Institute Pasteur), 127B : 195.
| Product Name | Modified Rappaport Vassiliadis Medium, Granulated |
|---|---|
| SKU | GM1137 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Rappaport F., Konforti N. and Navon B., 1956, J. Clin. Path., 9:261. |
| Customized Product Available | No |
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