Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Vaccine Production#CC293D
Intended Use
Recommended for mass cultivation of organisms for vaccine or toxin production.
Composition
| Ingredients | g/L |
|---|---|
| Peptone | 10.000 |
| HM infusion B from 500 g # | 10.000 |
| Sodium chloride | 5.000 |
| Agar | 25.000 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef infusion from
Directions
Suspend 50.0 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Coo to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
The principles of cultivation of bacteria were laid down in the late 1870's by Robert Koch. Since that time bacteriologists could study systematically the diseases caused by bacteria, isolate the causative agents in pure culture and make themselves familiar with their nature. With the aid of the culture technique they could produce therapeutic sera and prophylactic vaccines. Standard Infusion Agar supports luxuriant growth of a variety of bacteria. This medium is thus recommended for large-scale cultivation of bacteria for the purpose of vaccine and toxin production. Standard Infusion Agar has composition similar to HM Peptone B Agar (ATCC Medium 225) (1). Standard Infusion Broth, having a composition similar to Standard Infusion Agar is recommended as highly nutritious media for the cultivation of wide variety of microorganisms (2). Peptone and HM infusion B from provide nitrogen and carbon source, long chain amino acids sulphur, vitamins and other growth nutrients for luxuriant growth of organisms. Sodium chloride maintains the osmotic equilibrium.
Type of specimen
Isolated Microorganism
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 2.5% Agar gel.
Colour and Clarity of prepared medium
Yellow coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH: 7.5±0.2
pH
7.30-7.70
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant | >=70% |
| Salmonella Typhi ATCC 6539 | 50-100 | luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Standard Infusion Agar |
|---|---|
| SKU | M883 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Atlas R. M., 1993, Handbook of Microbiological Media, CRC Press. Inc. |
| Customized Product Available | No |
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Meat Infusion Agar, HiVeg™ (Revised as Standard Infusion Agar, HiVeg™)
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