Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Vaccine Production#CC293D
Composition
| Ingredients | Gms / Litre |
|---|---|
| Beef extract | 10.000 |
| Peptic digest of lean meat from | 500.000 |
| Sodium chloride | 5.000 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.6±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 45 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring in sterile Petri plates.
Principle and Interpretation
Standard Nutrient Agar is formulated as per the recommendation of APHA as a general purpose medium for the cultivation of non-fastidious organisms from water and wastewater, dairy and food products (1, 2).
Peptic digest of lean meat provides the amino acids and large chain peptides. Beef extract (meat infusion) provides water soluble substances like carbohydrates, vitamins, organic nitrogen compounds and salts (3). Sodium chloride maintains osmotic equilibrium.
Quality Control
Appearance
Yellowish brown coloured homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent gel forms in petri plates.
Reaction
Reaction of 4.5% w/v aqueous solution at 25°C. pH : 7.6±0.2
pH
7.40-7.80
Cultural Response
M877: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Escherichia coli ATCC 25922 | 50-100 | Good - luxuriant | >=70% |
| Staphylococcus aureus ATCC 25923 | 50-100 | Good - luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | Good - luxuriant | >=70% |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | Good - luxuriant | >=70% |
| Salmonella Typhi ATCC 6539 | 50-100 | Good - luxuriant | >=70% |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
| Product Name | Standard Nutrient Agar |
|---|---|
| SKU | M877 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Greenberg A. E., Trussell R. R. and Clesceri L. S. (Eds.), 1985, Standard Methods for the Examination of Water andWastewater, 16th ed., APHA, Washington, D.C. |
| Customized Product Available | No |
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