Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Vaccine Production#CC293D
Intended Use
Recommended for mass cultivation of Clostridia for enterotoxin production.
Composition
| Ingredients | g/L |
|---|---|
| HL infusion from 250g # | 5.000 |
| HM infusion B from 250g $ | 5.000 |
| Peptone, special | 20.000 |
| Sodium chloride | 5.000 |
| Dipotassium hydrogen phosphate | 4.000 |
| Final pH (at 25°C) | 8.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Liver, infusion from
$ Equivalent to Beef heart, infusion from
Directions
Suspend 39.0 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
E.T Medium is used for the mass cultivation of Clostridia for enterotoxin production. The media contains HL infusion from which due to its nutrients characteristics is recommended for cultivation of fastidious anaerobic bacteria such as Clostridia. The medium also contains HM infusion B, the muscle protein which provides amino acids and other nutrients.
Type of specimen
Clinical samples - Blood; Food samples.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2). For food samples, follow appropriate techniques for sample collection and processing as per guidelines (3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitation
- Further biochemical test must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to brownish yellow coloured with pink tinge homogeneous free flowing powder
Colour and Clarity of prepared medium
Amber coloured clear to slightly opalescent solution.
Reaction
Reaction of 3.9% aqueous solution at 25°C. pH: 8.4±0.2
pH
8.20-8.60
Cultural Response
Cultural characteristics observed under 10% Carbon dioxide (CO2) after an incubation at 35-37°C for 24-72 hours
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant |
| Clostridium botulinum ATCC 25763 | 50-100 | luxuriant |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant |
* - Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | E.T. Medium |
|---|---|
| SKU | M854 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | To be added |
| Customized Product Available | No |
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