Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Growth Media for Saccharomyces cerevisiae and Schizosaccharomyces pombe#CC293D
Intended Use
Complete Supplement Mixture (CSM) is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 10.00 |
| L-Arginine HC1 | 50.00 |
| L-Aspartic acid | 80.00 |
| L-Histidine HCl | 20.00 |
| L-Isoleucine | 50.00 |
| L-Leucine | 100.00 |
| L-Lysine HCl | 50.00 |
| L-Methionine | 20.00 |
| L-Phenylalanine | 50.00 |
| L-Threonine | 100.00 |
| L-Tryptophan | 50.00 |
| L-Tyrosine | 50.00 |
| Uracil | 20.00 |
| L-Valine | 140.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 790 mg in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Complete Supplement Mixture (CSM) is used as a dropout supplement for all strains of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.
Complete Supplement Mixture (CSM) is developed according to the formulae of Wickerham (1, 2) which supplies the essential nutritional elements for budding yeast cells. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of budding yeast. It is also the basis for many “Drop-Out” formulations for the selections of auxotrophic requirements and transformants.
Quality Control
Appearance of Powder: White to cream coloured, homogeneous, free flowing powder.
Colour and Clarity: Colourless, clear solution without any precipitate.
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Complete Supplement Mixture (CSM) |
|---|---|
| SKU | G100 |
| Customized Product Available | No |
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