Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Growth Media for Saccharomyces cerevisiae and Schizosaccharomyces pombe#CC293D
YEP Growth Agar is used as a base for making variation with an alternate carbon sources, for the growth of Saccharomyces cerevisiae.
Composition** :
| Ingredients | Grams/Litre |
|---|---|
| Peptone | 20.00 |
| Yeast extract | 10.00 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions:
Suspend 45 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle and Interpretation :
YEP Growth Agar is used as a base for making variation with an alternate carbon sources, for the growth of Saccharomyces cerevisiae.
Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. They are chemoorganotrophs as they utilize organic compounds as a source of energy.
YEP Growth Agar is used for the maintenance and propagation of yeasts including S. cerevisiae in various molecular microbiology procedures (1, 2). This media functions as a complete medium for yeast growth and it contains yeast extract, peptone and agar. Yeast extract supplies B-complex vitamins and it contains all the amino acids necessary for growth. Peptone acts as the source of nitrogen, vitamins and minerals. This medium supports the vigorous growth of wild type as well as mutant strains of all kinds of budding yeast.
Quality Control :
Appearance of Powder : Cream to yellow coloured, homogeneous, free flowing powder.
Gelling:
Firm, comparable with 1.5% Agar gel
Colour and Clarity :
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response :
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC9763 | good-luxuriant |
Storage and Shelf-life :
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | YEP Growth Agar |
|---|---|
| SKU | G040 |
| Customized Product Available | No |
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