Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Growth Media for Saccharomyces cerevisiae and Schizosaccharomyces pombe#CC293D
Intended Use
YEP Growth Medium is used as a base for making variation with an alternate carbon sources, for the growth of Saccharomyces cerevisiae.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Peptone | 20.00 |
| Yeast extract | 10.00 |
| Final pH (at 25°C) | 6.5 ± 0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
YEP Growth Medium is used as a base for making variation with an alternate carbon sources, for the growth of Saccharomyces cerevisiae. Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. They are chemoorganotrophs as they utilize organic compounds as a source of energy. YEP Growth Medium is used for the maintenance and propagation of yeasts including S. cerevisiae in various molecular microbiology procedures (1, 2). YEP functions as a complete medium for yeast growth and it contains yeast extract and peptone. Yeast extract supplies B-complex vitamins and it contains all the amino acids necessary for growth. Peptone acts as the source of nitrogen, vitamins and minerals. This medium supports the vigorous growth of wild type as well as mutant strains of all kinds of budding yeast.
Quality Control
Appearance of Powder : Cream to light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity : Light amber coloured, clear solution without any precipitate.
Reaction
Reaction of 3.0% w/v aqueous solution is pH 6.5 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
Growth Promoting Properties
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | YEP Growth Medium |
|---|---|
| SKU | G039 |
| Customized Product Available | No |
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