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Synthetic Complete Supplement Mixture (SC) w/o URA

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SKU:
G155
Synthetic Complete Supplement Mixture for amino acids to prepare appropiate media for selection of URA3 auxotrophic strains and transformants

Intended Use

Synthetic Complete Supplement Mixture (SC) w/o URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae.

Composition

Ingredients Milligrams/Litre
Adenine21.00
L-Alanine85.60
L-Arginine HCI85.60
L-Asparagine85.60
L-Aspartic acid85.60
L-Cysteine HCI85.60
Glutamine85.60
L-Glutamic acid85.60
Glycine85.60
L-Histidine HCl85.60
Myo-Inositol85.60
L-Isoleucine85.60
L-Leucine173.40
L-Lysine HCl85.60
L-Methionine85.60
L-Phenylalanine85.60
L-Proline85.60
L-Serine85.60
L-Threonine85.60
L-Tryptophan85.60
L-Tyrosine85.60
L-Valine85.60

Formula adjusted, standardized to suit performance parameters

Directions

Suspend 1.91 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.

Principle and Interpretation

Synthetic Complete Supplement Mixture (SC) w/o URA supplies the essential nutritional elements for budding yeast cells. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae but it does not contain uracil, the pyrimidine derivative. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), Ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of uracil prototrophs and wild type strains of S. cerevisiae but uracil auxotroph cannot grow in this media. Therefore, SC w/o URA can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.

Quality Control

Appearance of Powder: White to off-white coloured, homogeneous, free flowing powder.

Colour and Clarity: Colourless clear solution without any precipitate.

Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.

Organisms (ATCC) Growth
Saccharomyces cerevisiae ATCC 9763 good-luxuriant

Storage and Shelf-life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Do not use if package is damaged

More Information
Product Name Synthetic Complete Supplement Mixture (SC) w/o URA
SKU G155
Customized Product Available No
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