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Synthetic Complete Supplement Mixture (SC) w/o TRP

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SKU:
G154
Synthetic Complete Supplement Mixture for amino acids to prepare appropiate media for selection of TRP1 auxotrophic strains and transformants

Composition

Ingredients Milligrams/Litre
Adenine 21.00
L-Alanine 85.60
L-Arginine HCl 85.60
L-Asparagine 85.60
L-Aspartic acid 85.60
L-Cysteine HCl 85.60
Glutamine 85.60
L-Glutamic acid 85.60
Glycine 85.60
L-Histidine HCl 85.60
Myo-Inositol 85.60
L-Isoleucine 85.60
L-Leucine 173.40
L-Lysine HCl 85.60
L-Methionine 85.60
L-Phenylalanine 85.60
L-Proline 85.60
L-Serine 85.60
L-Threonine 85.60
L-Tyrosine 85.60
Uracil 85.60
L-Valine 85.60

** Formula adjusted, standardized to suit performance parameters

Directions

Suspend 1.91 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.

Principle and Interpretation

Synthetic Complete Supplement Mixture (SC) w/o TRP is used as a dropout supplement for all strains of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.

Synthetic Complete Supplement Mixture (SC) w/o TRP supplies the essential nutritional elements for budding yeast cells. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae except tryptophan. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of tryptophan prototrophs and wild type strains of S. cerevisiae but tryptophan auxotroph cannot grow in this media. Therefore, SC w/o TRP can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.

Quality Control

Appearance of Powder : White to light yellow coloured, homogeneous, free flowing powder.

Colour and Clarity : Colourless to pale yellow coloured, clear solution without any precipitate.

Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.

Organisms (ATCC) Growth
Saccharomyces cerevisiae ATCC 9763 good-luxuriant

Storage and Shelf-life

Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.

More Information
Product Name Synthetic Complete Supplement Mixture (SC) w/o TRP
SKU G154
Customized Product Available No
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