Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Estimating Counts of Fungi - Yeasts and Molds#CC293D
Composition
| Ingredients | Gms / Litre |
|---|---|
| Potatoes, infusion from | 200.000 |
| Dextrose | 20.000 |
| Final pH (at 25°C) | 5.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 24.0 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.
Principle And Interpretation
Potato Dextrose Broth is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Broth is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (4).
Potato infusion and dextrose promote luxuriant fungal growth. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided.
Quality Control
Appearance
Off-white to yellow coloured granular medium.
Colour and Clarity of prepared medium
Light amber coloured clear to slightly opalescent solution in tubes
Reaction
Reaction of 2.4% w/v aqueous solution at 25°C. pH : 5.1±0.2
pH
4.90-5.30
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for 4-5 days.
| Organism | Inoculum (CFU) | Growth | Ascospore formation |
|---|---|---|---|
| #Aspergillus brasiliensis ATCC 16404 (00053*) | 50-100 | luxuriant | negative |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant | negative |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | 50-100 | luxuriant | positive |
Key:- # Formerly known as Aspergillus niger
Key:- * Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 10-30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Potato Dextrose Broth, Granulated |
|---|---|
| SKU | GM403 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. 2.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC. 3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 4.MacFaddin J. F., 1985, Media for the Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1, Williamsand Wilkins, Baltimore |
| Customized Product Available | No |
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