Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Controlling Fermentation Processes#CC293D
Composition
| Ingredients | Gms / Litre |
|---|---|
| Malt extract | 15.000 |
| Peptic digest of animal tissue | 0.780 |
| Maltose | 12.750 |
| Dipotassium phosphate | 1.000 |
| Ammonium chloride | 1.000 |
| Dextrin | 2.750 |
| Final pH (at 25°C) | 4.8±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 33.28 grams in 1000 ml distilled water containing 2.35 grams of glycerol. Heat if necessary, to dissolve the medium completely. Mix well and dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Wort Broth is formulated as described by Parfitt (1) for the cultivation of fungi especially yeasts in syrups and butter. Wort Broth is especially designed to propagate the multiplication of yeasts and often it has been employed as a semi-selective or enrichment medium. Yeast grows well in culture media containing dextrin or maltose in acidic environment.
In this medium peptic digest of animal tissue and malt extract provide nitrogenous and other nutrients for the growth of yeasts. Dextrin and maltose are fermentable carbohydrates. The high acidic pH inhibits many bacteria. Phosphate buffers the medium.
Quality Control
Appearance
Light yellow to brownish yellow coloured granular medium
Colour and Clarity of prepared medium
Yellow coloured opalescent solution in tubes that may contain a flocculent precipitate.
Reaction
Reaction of 3.33% w/v aqueous solution at 25°C. pH : 4.8±0.2
pH
4.60-5.00
Cultural Response
Cultural characteristics observed with added glycerol after an incubation at 25-30°C for 40-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant |
| Candida albicans ATCC 10231 | 50-100 | luxuriant |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant |
| Saccharomyces uvarum ATCC 28098 | 50-100 | luxuriant |
*-Formerly known as Aspergillus niger
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Wort Broth, Granulated |
|---|---|
| SKU | GM333 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Parfitt, 1933., J. Dairy Sci., 19 : 141. |
| Customized Product Available | No |
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