Sabouraud Maltose Broth

Intended Use

Recommended for propagation of yeasts and moulds, particularly the parasitic fungi concerned with skin and scalp lesions.

Composition

Formula adjusted, standardized to suit performance parameters

Directions

Suspend 50 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Mix well and dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C.

Principle And Interpretation

Fungi were among the first microorganisms recognized because some of the fruiting structures, such as the mushrooms, are large enough to be seen without a microscope. Fungi can be grouped simply on the basis of morphology as either yeasts or moulds (1). Sabouraud Maltose Broth was formulated by Sabouraud (2) and is used for the isolation and differentiation of yeast and moulds (3,4,5).

Mycological peptone provides nitrogen, vitamins, minerals, amino acids and growth factors. Maltose provides an energy source for the growth of microorganisms. The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens (6). The acid reaction of the final medium is inhibitory to a large number of bacteria making it particularly useful for cultivating fungi and aciduric microorganisms. For isolation of fungi from contaminated specimens, a selective medium should be inoculated simultaneously. Incubate cultures for 4 to 6 weeks before reporting as negative.

Type of specimen

Clinical samples - skin and scalp lesions, etc.

Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7).

After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

• 1. Further biochemical and serological tests must be carried out for further identification.
• 2. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
• 3. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance

Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium

Light amber coloured clear solution in tubes

Reaction

Reaction of 5.0% w/v aqueous solution at 25°C. pH: 5.6±0.2

pH

5.40-5.80

Cultural Response

Cultural characteristics observed after an incubation at 25 - 30°C for 48-72 hours.(Incubate Trichophyton species for upto 7 days)

Key: *Corresponding WDCM numbers. #- Formerly known as Aspergillus niger

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).

Reference

1. 1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
2. 2. Sabouraud R., 1892, Ann. Dermatol. Syphil. 3: 1061.Davidson and Dowding, 1932, Arch. Dermatol. Syphilol. 26:660.
3. 3. Davidson, Dowding and Buller. 1932. Can. J. Res. 6:1.
4. 4. Frank L. S., 1932, Arch. Dermatol. Syphilol., 26: 457
5. 5. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition.
6. 6. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology, 11th Edition. Vol. 1.