Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Rhizobium and Other Root Nodulating Bacteria#CC293D
Intended Use
Highly nutritious medium that can support growth of fastidious microorganism.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 20.000 |
| Dextrose (Glucose) | 10.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to45-50°C. Mix well & pour in sterile Petri plates.
Principle And Interpretation
Glucose Peptone Agar is recommended for general cultivation of wide variety of microorganisms. As it is rich in nutrients can also serve as excellent basal medium for glucose blood agar. With addition of suitable indicator, this medium can be used for the detection and cultivation of thermophilic organisms, associated with flat sour spoilage in Canned goods. Agrobacterium species can also grow abundantly on media containing dextrose as carbohydrate source. Glucose peptone Agar with addition of Bromocresol purple (1% alcoholic solution) is suitable for cultivation of root nodulating bacteria (5). Peptone provides nitrogenous nutrients especially amino acids, and peptides. The presence of sodium chloride helps to maintain the osmotic balance. Dextrose serves as fermentable carbohydrate source and carbon source.
Type of specimen
Food samples; Soil samples
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (4).
For soil samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(5)
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow coloured homogeneous free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel.
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates.
Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Agrobacterium tumefaciens ATCC 23308 | 50-100 | Good - luxuriant | >=70% |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | Good - luxuriant | >=70% |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | Good - luxuriant | >=70% |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | Good - luxuriant | >=70% |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | Good - luxuriant | >=70% |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Glucose Peptone Agar |
|---|---|
| SKU | M758 |
| Customized Product Available | No |
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