Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Rhizobium and Other Root Nodulating Bacteria#CC293D
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Sucrose | 20.000 |
| Dipotassium hydrogen phosphate | 1.000 |
| Magnesium sulphate | 0.500 |
| Sodium chloride | 0.500 |
| Ferrous sulphate | 0.100 |
| Sodium molybdate | 0.005 |
| Calcium carbonate | 2.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 24.1 grams in 1000 ml distilled water. Heat just to boiling. Mix well and dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Note: Due to presence of calcium carbonate, the medium forms opalescent solution with white precipitate.
Principle And Interpretation
Nitrogen-fixing organisms are free-living bacteria, which grow well on a nitrogen-free medium. These bacteria utilize atmospheric nitrogen gas for their cell protein synthesis. This cell protein is then mineralized in soil after the death of the cells thereby contributing towards the nitrogen availability of the crop plants (1). Nitrogen fixing bacteria enter into symbiosis only with leguminous plants, by infecting their roots and forming nodules on them. Jensens Broth is formulated according to Jensen and is recommended for detection and cultivation of nitrogen fixing bacteria (2).
Sucrose acts as the energy source. Sodium molybdate in the media increases the fixation of nitrogen (3). Dipotassium hydrogen phosphate buffers the medium well. Sodium chloride maintains osmotic equilibrium of the medium. Calcium stimulates nodulation when present as chloride or sulphate.
Quality Control
Appearance
White to cream coloured granular medium.
Colour and Clarity of prepared medium
Cream coloured, slightly opalescent solution with slight precipitate in tubes
Cultural Response
Cultural characteristics observed after an incubation at 25-30°C for upto 8 days.
| Organism | Growth |
|---|---|
| Rhizobium leguminosarum ATCC 10004 | luxuriant |
| Rhizobium meliloti ATCC 9930 | luxuriant |
| Rhizopus oryzae ATCC 9363 | luxuriant |
Storage and Shelf Life
On receipt store between 10- 30°C in tightly closed container and use freshly prepared medium. Use before expiry period on the label.
| Product Name | Jensen’s Broth, Granulated |
|---|---|
| SKU | GM973 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1.Subba Rao N. S., 1977, In: Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi, Pages254-255.2.Jensen. H. L., 1942, Pro Line Soc. N.S.W., 57,205-212.3.Ranganayaki S., Mohan C., Ally Z., 1981; 21 (8): 607-10. |
| Customized Product Available | No |
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