Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Bacteria#CC293D
Intended Use
Recommended for isolation of yeasts from soil specimens.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Peptone | 10.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 20.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.0 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Yeasts are unicellular organisms that reproduce by budding. Their microscopic and morphological features usually appear similar for different genera and are not particularly helpful in their isolation in pure culture. Glucose Yeast Peptone Agar is formulated as described by Subba Rao (4) with a slight modification in agar concentration for isolating yeasts from soil specimens. This is a highly nutritious medium, which may be used not only for isolating yeasts but also for isolating some fastidious microorganisms. Yeasts grow well on a minimal medium containing only dextrose and salts. The addition of protein and yeast cell extract hydrolysates allows faster growth so that during exponential or log-phase growth, doubling time of 90 minutes is observed (1).
Peptone provides nitrogenous nutrients especially the amino acids and peptides and yeast extract supplies vitamin B complex. Dextrose is the readily available source of energy and a good carbohydrate source for yeasts.
Type of specimen
Soil samples.
Specimen Collection and Handling
For soil samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (4). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some species may show poor growth due to nutritional variations.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light to medium amber coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.0% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH: 6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Recovery |
|---|---|---|---|
| Saccharomyces cerevisiae ATCC 9763 (00058*) |
50-100 | luxuriant | >=70% |
Key: * Corresponding WDCM numbers.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | Glucose Yeast Peptone Agar |
|---|---|
| SKU | M757 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Subba Rao N. S., 1977, Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi. 2.Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl, 1994, Current Protocols in Molecular Biology, CurrentProtocols, Brooklyn, N.Y. |
| Customized Product Available | No |
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