Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Actinomycetes#CC293D
Intended Use
Recommended for the isolation of Actinomycetes and other fungi from clinical and non-clinical samples.
Composition**
| Ingredients | g/L |
|---|---|
| Starch, soluble | 15.000 |
| Yeast extract | 4.000 |
| Dipotassium hydrogen phosphate | 1.000 |
| Magnesium sulphate | 0.500 |
| Agar | 20.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40.5 grams in 1000 ml purified / distilled water. If desired, half strength medium can be prepared using 20.25 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Fungi were among the first microorganisms recognized because some of the fruiting structures, such as the mushrooms, are large enough to be seen without a microscope. Fungi are extremely successful organisms, as evidenced by their ubiquity in nature. They are an important component in the energy cycle where they function as decomposers (1). Actinomycetes are distributed worldwide, found as part of the indigenous microflora found in soil, mud etc. and also as parasites of humans and other animals (1).
Emerson YSS (Yeast Soluble Starch) Agar recommended for the isolation of Actinomycetes and other fungi was formulated by Emerson (2). This medium was used in half strength by Emerson and Wilson (3) to obtain single germlings from zygotes or zoospores.
Yeast extract serves as a source of B-complex vitamins, amino acids and essential nutrients. Soluble starch serves as a source of energy and carbon. It also neutralizes the toxic metabolites formed. Phosphates buffer the medium whereas magnesium sulphate acts as a source of ions and sulphates. Standard reference for the isolation, cultivation and colony characteristics of various fungi should be followed.
Type of specimen
Clinical samples- oral swab, intestinal swab, etc. Soil samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Further biochemical and serological tests must be carried out for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to pink homogeneous free flowing powder
Gelling
Firm, comparable with 2.0% agar gel.
Colour and Clarity of prepared medium
Light to medium amber coloured, opalescent gel with a slight flocculant precipitate forms in Petri plates
Reaction
Reaction of 4.05% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 30°C for 40-72 hours.
| Organism | Growth |
|---|---|
| # Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | luxuriant |
| Saccharomyces uvarum ATCC 28098 | luxuriant |
Key: (*) Corresponding WDCM numbers, (#) Formerly known as Aspergillus niger.
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Emerson YSS Agar |
|---|---|
| SKU | M773 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 2.Emerson, 1941, Lloydia, 4:77. 3.Emerson and Wilson, 1954, Mycologia, 46:393. |
| Customized Product Available | No |
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Actinomyces Agar
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