Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Masophilic Sporeforming - Anaerobic#CC293D
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Casein enzymic hydrolysate | 10.000 |
| Glucose | 5.000 |
| Yeast extract | 1.000 |
| Dipotassium phosphate | 1.250 |
Final pH (at 25°C): 6.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 17.25 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense in sterile test tubes.
Principle And Interpretation
Tryptone Glucose Yeast Extract Broth is recommended by APHA (1) for the enumeration of microorganisms during microbiological examination of food materials by MPN technique.
Casein enzymic hydrolysate, yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Glucose is the energy source whereas dipotassium phosphate buffers the medium.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear solution in tubes.
Reaction
Reaction of 1.72% w/v aqueous solution at 25°C. pH : 6.8±0.2
pH
6.60-7.00
Cultural Response
M952: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant |
| Enterococcus faecalis ATCC 29212 | 50-100 | luxuriant |
| Lactobacillus casei ATCC 9595 | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Reference
- Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C.
| Product Name | Tryptone Glucose Yeast Extract Broth |
|---|---|
| SKU | M952 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. |
| Customized Product Available | No |
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