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Masophilic Sporeforming - Anaerobic#CC293D
Intended Use
Recommended for detection and cultivation of mesophilic anaerobic spore formers in specimens collected from food processing plants.
Composition
| Ingredients | g / L |
|---|---|
| Peptone | 20.000 |
| Yeast extract | 3.000 |
| Bromocresol purple | 0.040 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23.04 grams in 1000 ml purified / distilled water. Heat if necessary to dissolve the medium completely. Dispense 18-20 ml aliquots into 18 x 150 mm screw capped test tubes. Add 8-10 untreated Alaska seed peas and let the tubes stand for 1 hour to effect hydration. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
The mesophilic spore-forming anaerobes belong to the genus Clostridium and are widely distributed in nature. Clostridial species are highly heat resistant and are able to grow in the absence of oxygen. Clostridial growth range covers the temperature of the normal storage of canned and other processed foods including refrigerated storage of cured meats and hence these anaerobes are important in the spoilage of low-acid foods packed in hermetically sealed containers. PE-2 Medium is prepared as per the formulation described by Folinazzo and Troy (1) and recommended by APHA (2) for detection and cultivation of mesophilic anaerobic spore-formers in specimens from food processing plants. These organisms mainly include the genus Clostridium.
Peptone and yeast extract provide nitrogenous compounds, vitamin B complex and trace ingredients required for the growth of clostridia. Addition of untreated alaska seed peas creates anaerobic conditions in the medium.
Type of specimen
Food samples- Dehydrated vegetables and spices
Specimen Collection and Handling
Prepared samples of heated sugar, dehydrated vegetables and spices are cultured by taking 20 ml portions of these heated substances and dividing equally among 6 tubes of freshly heated culture medium. Incubate the cultures at 30-35°C for 72 hours or upto 7 days if desired as some spores germinate slowly (2).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Purple coloured clear to slightly opalescent solution over alaska seeds.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth |
|---|---|---|
| Clostridium botulinum ATCC 25763 |
50-100 | good-luxuriant |
| Clostridium sporogenes ATCC 11437 |
50-100 | good-luxuriant |
| #Thermoanaerobacterium thermosaccharolyticum ATCC 7956 (00135*) |
50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
# Formerly known as Clostridium thermosaccharolyticum
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | PE-2 Medium |
|---|---|
| SKU | M611 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Folinazzo J. F. and Troy V. S., 1954, Food Technol., 8:280. 2.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. |
| Customized Product Available | No |
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