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Yeast Extract Rose Bengal Broth Base

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SKU:
M955
For the cold enrichment for the recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from food samples as per APHA.

Composition

Ingredients Gms / Litre
Yeast extract 5.000
Disodium phosphate 17.250
Bile salts 2.000
Sodium chloride 1.000
Magnesium sulphate 0.010
Sodium pyruvate 1.000
Rose bengal 0.040
Final pH (at 25°C) 7.9±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 26.3 grams in 900 ml distilled water. Heat if necessary, to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 20-25°C and aseptically add 100 ml of 4% filter sterilized sorbose solution. Mix well and dispense aseptically as desired.

Principle And Interpretation

Yersinioses are zoonotic infections that usually affect rodents, small animals and birds, while humans are accidental hosts.

Yersinia enterocolitica is a significant and invasive enteric pathogen that causes several well-recognized diseases, especially in younger persons, and several uncommon post-infection syndromes (1). Yersinia pseudotuberculosis causes a zoonotic disease with its natural reservoir being rodents, wild animals and game birds (2). Yeast Extract Rose Bengal Broth is formulated as recommended in APHA for enrichment of Yersinia species from foods (3), using the cold enrichment method. Yersinia species are psychrotropic and therefore grow at 4°C. Yeast extract provides essential nutrients. Bile salts inhibit gram-positive organisms. Various salts help in cold enrichment of Yersinia species.

Y. enterocolitica and Y. pseudotuberculosis can grow at 4°C, so primary enrichment is carried out in this medium for 9 days at 4°C or 3 days at 10°C (4). From this enrichment the organisms are further enriched in secondary selective enrichment medium such as PSTA Broth (M940) and then isolated by streaking onto selective plating media such as Yersinia Selective Agar (M843), SS Agar (M108) and MacConkey Agar (M082).

Quality Control

Appearance
Light yellow to pink homogeneous free flowing powder

Colour and Clarity of prepared medium
Reddish pink coloured clear solution without any precipitate.

Reaction
Reaction of the medium (2.63% w/v 90ml Base + 10 ml of 4% w/v sorbose) at 25°C. pH: 7.9±0.2

pH
7.70-8.10

Cultural Response

Cultural characteristics observed after incubation at 4°C for 9-10 days or at 10°C for 3 days with added sorbose solution.

Cultural Response

Organism Inoculum (CFU) Recovery
Yersinia enterocolitica ATCC 27729 50-100 good-luxuriant
Yersinia pseudotuberculosis ATCC 29833 50-100 good-luxuriant

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.

Reference

  1. Cover T. L. and Aber R. C., 1989, N. Engl. J. Med. 321:16-24
  2. Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
  3. Speck M. L., (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd Ed., APHA, Washington, D.C.
  4. Schiemann D. A., 1982, Appl. Environ. Microbiol., 43:14.
More Information
Product Name Yeast Extract Rose Bengal Broth Base
SKU M955
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. Cover T. L. and Aber R. C., 1989, N. Engl. J. Med. 321:16-242.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.3.Speck M. L., (Ed.), 1984, Compendium of Methods for the Microbiological Examination of Foods, 2nd Ed., APHA,Washington, D.C.4.Schiemann D. A., 1982, Appl. Environ. Microbiol., 43:14.
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