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Listeria#CC293D
Rhamnose Broth is recommended for demonstration of rhamnose fermentation (Listeria monocytogenes).
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Meat extract | 1.000 |
| Meat peptone | 10.000 |
| Sodium chloride | 5.000 |
| Bromo cresol purple | 0.020 |
Final pH (at 25°C): 6.8±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 16.02 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes containing inverted Durham's tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Aseptically add filter sterilized rhamnose solution (final concentration of 1%) to sterile media.
Principle And Interpretation
Only Listeria monocytogenes among the Listeria species is reported to cause infection in humans. In human adults, L. monocytogenes primarily causes meningitis, encephalitis or septicemia. The tropism of L. monocytogenes for the central nervous system leads to severe disease, often with high mortality or with neurologic disorders among survivors (1).
Rhamnose Broth Base is formulated for demonstration of rhamnose fermentation. Meat extract and Meat Peptone provides essential growth nutrients for bacterial metabolism. Bromocresol purple acts as pH indicator which turns yellow under acidic condition. Sodium chloride maintains osmotic equilibrium. Gas formation is seen in Durham's tubes.
Quality Control
Appearance: Light yellow to bluish grey coloured homogeneous free flowing powder
Colour and Clarity of prepared medium: Purple coloured clear solution in tube
Reaction: Reaction of 1.6% w/v aqueous solution at 25°C. pH: 6.8±0.2
pH: 6.60-7.00
Cultural Response
M1364: Cultural characterisitcs observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Acid | Gas |
|---|---|---|---|---|
| Listeria grayi ATCC 19120 | 50-100 | luxuriant | Positive reaction, yellow colour | Negative reaction |
| Listeria innocua ATCC 33090 | 50-100 | luxuriant | Positive reaction, yellow colour | Negative reaction |
| Listeria ivanovii ATCC 19119 | 50-100 | luxuriant | Negative reaction, no colour change | Negative reaction |
| Listeria monocytogenes ATCC 19111 | 50-100 | luxuriant | Positive reaction, yellow colour | Negative reaction |
| Listeria monocytogenes ATCC 19112 | 50-100 | luxuriant | Positive reaction, yellow colour | Negative reaction |
| Listeria seeligeri ATCC 35967 | 50-100 | luxuriant | Negative reaction, no colour change | Negative reaction |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology, 8th Ed., American Society for Microbiology, Washington, D.C.
- Schweizersches Lebensmittelbuch, Jan. 201. Chapter 56.
| Product Name | Rhamnose Broth |
|---|---|
| SKU | M1364 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Eds.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C.2.Schweizersches Lebensmittelbuch, Jan. 201. Chapter 56. |
| Customized Product Available | No |
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