RS Medium Base

RS Medium Base is used for selective isolation, cultivation and presumptive identification of Aeromonas hydrophila from clinical and non-clinical samples.It is used for selective isolation and presumptive identification of Aeromonas hydrophila and other gram-negative bacteria based on their ability to decarboxylate lysine and ornithine, maltose fermentation and H2S production. Yeast extract acts as a source of nutrients. Sodium thiosulphate, L-cysteine hydrochloride and ferric ammonium citrate are the indicators of H2S production. Maltose is fermentable carbohydrate source. Bromothymol blue is the pH indicator. Sodium deoxycholate and novobiocin inhibit gram-positive bacteria and Vibrio species. Aeromonas hydrophila show yellow coloured colonies due to maltose fermentation, Lysine and ornithine decarboxylation negative and do not produce H2S.