Clostridium Brazier Agar Base

Clostridium Brazier Agar Base is a selective media for isolation and differentiation of Clostridium difficile with added supplements. The selective agents in Clostridium difficile supplement (FD010), D-cycloserine and cefoxitin used in this medium inhibits the growth of the majority of Enterobacteriaceae and also Enterococcus faecalis, Staphylococci, gram negative anaerobic bacilli and Clostridium species other than C. difficile which may be found abundantly in samples. The Egg Yolk Emulsion (FD045) added to the medium helps to differentiate C. difficile from lecithinase positive Clostridia. Addition of lysed horse blood to the base enhances recognition of colony fluorescence when cultures are examined using UV light. Clostridium difficile appears as greyish-white, opaque flat colonies and are lecithinase negative.