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HiEncap™ Soyabean Casein Digest Medium (HiEncap™ Tryptone Soya Broth)
A general purpose medium used for cultivation of a wide variety of microorganismsn and sterility testing of moulds and lower bacteria.
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Intended Use
Recommended a general purpose medium used for cultivation of a wide variety of microorganisms and sterility testing of moulds and lower bacteria.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Each capsule contains 7.5 grams of media. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) purified or distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Note: If any fibres are observed in the solution, it is recommended to filter the solution by using a 0.22 micron filter to eliminate the possibility of presence of fibres.
Principle And Interpretation
Soyabean Casein Digest Medium is recommended by various pharmacopeias as a sterility testing and as a microbial limit testing medium (2,5,6). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (1). The combination of tryptone and soya peptone makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and Dipotassium hydrogen phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium.
Type of specimen
Pharmaceutical samples
Specimen Collection and Handling
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per pharmaceutical guidelines (6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred to individual safety data sheets.
Limitations
- Biochemical characterization is necessary to be performed on colonies from pure cultures for further identification.
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Gelatin capsule containing cream to yellow coloured granulated media
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate.
Quantity: Each capsule contains 7.5 grams of medium sufficient for 250 ml media
Reaction: pH of 3.0% w/v aqueous solution at 25°C . pH : 7.3±0.2
pH: 7.10-7.50
Cultural Response: Cultural characteristics observed after an incubation at 30-35°C for <= 3 days for Bacteria and at 20-25°C for <=5 days for fungii
Growth promoting
| Organism | Growth | Incubation period | Inoculum (CFU) | Incubation temperature |
|---|---|---|---|---|
| Escherichia coli ATCC 8739 (00012*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Candida albicans ATCC 2091 (00055*) | luxuriant | <=5 d | 50-100 | 30-35 °C |
| Streptococcus pneumoniae ATCC 6305 | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Salmonella Abony NCTC 6017 (00029*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Micrococcus luteus ATCC 9341 | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Escherichia coli NCTC 9002 | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | luxuriant | 18-24 hrs | 50-100 | 30-35 °C |
Sterility Testing- Growth promotion+Validation
| Organism | Growth | Incubation period | Inoculum (CFU) | Incubation temperature |
|---|---|---|---|---|
| Micrococcus luteus ATCC 9341 | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Salmonella Abony NCTC 6017 (00029*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Candida albicans ATCC 10231 (00054*) | luxuriant | <=5 d | 50-100 | 30-35 °C |
| #Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant | <=5 d | 50-100 | 30-35 °C |
| Escherichia coli ATCC 8739 (00012*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Escherichia coli NCTC 9002 | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Bacillus subtilis subsp. spizizenii ATCC 6633 (00003*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Streptococcus pneumoniae ATCC 6305 | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Staphylococcus aureus ATCC 6538 (00032*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Pseudomonas aeruginosa ATCC 9027 (00026*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | <=3 d | 50-100 | 20-25 °C |
Key:(*) Corresponding WDCM numbers #Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | HiEncap™ Soyabean Casein Digest Medium (HiEncap™ Tryptone Soya Broth) |
|---|---|
| SKU | EC011CCL |
| Application/ Industry | Clinical |
| Product Type | HiEncap™ |
| Physical Form | Granulated Media in Gelatin Capsule |
| Origin | Animal |
| Packaging type | HDPE Jar |
| References | 1.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams& Wilkins, Baltimore, M.d. 2. The United States Pharmacopeia, 2008, USP31/NF26, The United States Pharmacopeial Convention, Rockville, MD. 3. Indian Pharmacopeia, 2007, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India. 4. Forbes B. A., Sahm D. F. and Weissfeld A. S., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc. St.Louis, Mo. |
| Customized Product Available | No |
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