Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Anaerobic HiVeg™ Agar (Brewer)
As low as
$221.10
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Availability: In stock
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SKU:
MV491
For the isolation and sensitivity testing of anaerobic and microaerophilic organisms and study of colonial morphology.
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Intended Use
Recommended for the isolation and sensitivity testing of anaerobic and microaerophilic organisms and study of colonial morphology.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg® peptone No. 3 | 10.000 |
| HiVeg® hydrolysate | 5.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 10.000 |
| Sodium chloride | 5.000 |
| Sodium thioglycollate | 2.000 |
| Sodium formaldehyde sulphoxylate | 1.000 |
| Resazurin | 0.002 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 53.0 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Brewer (1) devised this medium for use with Brewer anaerobic cover to permit surface growth of anaerobes and microaerophiles on agar without the use of anaerobic jar. This medium is suitable for isolation of facultative and obligate anaerobes and for the study of colonial morphology as colonies can be readily seen on the light coloured agar and are easily accessible (2,3). Dispense 50-60 ml medium per 95 x 20 mm plate. For best results, use porous tops for the plates during solidification to obtain a dry surface. Inoculation can be done by streaking or smearing. After inoculation of the medium, cover with Brewer Anaerobic Petri dish cover. The sealing ring inside the cover should make a perfect contact with the medium and must not be broken before the end of the incubation period. When standard plates are used, dispense 0.1 to 1.0 ml of inoculum into plates and mix 20-25 ml of sterile medium. Anaerobic HiVeg® Agar (Brewer) is prepared by completely replacing animal based peptone with vegetable peptones to avoid BSE/TSE risks associate with animal peptones. HiVeg® peptone No. 3, HiVeg® hydrolysate and yeast extract provides nitrogen, carbon, long chain amino acids, vitamins and other essential growth nutrients. Dextrose is a carbohydrate source. This medium contains sodium thioglycollate and sodium formaldehyde sulphoxylate that provide adequate anaerobiosis, which is indicated by resazurin present in the medium. Resazurin imparts pink colour to the medium in presence of oxygen.
Type of specimen
Please add specimens
Specimen Collection and Handling
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Ensure that the clinical samples are properly transported under anaerobic conditions.
- Proper anaerobic conditions must be maintained for optimal recovery of organisms
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates that becomes red due to aeration on standing.
Reaction: Reaction of 5.3% w/v aqueous solution at 25°C. pH: 7.2±0.2
pH: 7.00-7.40
Cultural Response
Cultural characteristics observed under anaerobic condition, after an incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Clostridium botulinum ATCC 19397 | 50-100 | luxuriant | >=50% |
| Clostridium perfringens ATCC 12924 | 50-100 | luxuriant | >=50% |
| Clostridium sporogenes ATCC 11437 | 50-100 | luxuriant | >=50% |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Anaerobic HiVeg™ Agar (Brewer) |
|---|---|
| SKU | MV491 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Brewer, 1942, Science, 95, 587. 2.Isenberg (Ed.), 1992, Clinical Microbiology Procedures Handbook, American Society for Microbiology, Washington, D.C. 3.Baron E. J., Peterson and Finegold S. M., Bailey & Scotts Diagnostic Microbiology, 9th Ed., 1994, Mosby-Year Book, Inc.,St. Louis, Mo.4JorgensenJ.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manual of Clinical Microbiology,11th Edition. Vol. 1. . |
| Customized Product Available | No |
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