Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Rogosa SL HiVeg™ Broth
As low as
$201.30
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MV407
A selective medium for cultivation of oral, vaginal and faecal Lactobacilli.
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Intended Use:
Recommended for selective cultivation of all Lactobacilli.
Composition**
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate No. 1 | 10.000 |
| Yeast extract | 5.000 |
| Dextrose (Glucose) | 10.000 |
| Arabinose | 5.000 |
| Saccharose (Sucrose) | 5.000 |
| Sodium acetate | 15.000 |
| Ammonium citrate | 2.000 |
| Potassium dihydrogen phosphate | 6.000 |
| Magnesium sulphate | 0.570 |
| Manganese sulphate | 0.120 |
| Ferrous sulphate | 0.030 |
| Polysorbate 80 (Tween 80) | 1.000 |
| Final pH after addition of glacial acetic acid( at 25°C) | 5.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 59.72 grams in 1000 ml purified/distilled water. Adjust the pH of the medium with glacial acetic acid approximately (1.32 ml). Heat to boiling (90-100°C) for 3 minutes with frequent agitation to dissolve the medium completely. DO NOT AUTOCLAVE. Mix thoroughly and distribute into sterile culture tubes or flasks as desired.
Principle And Interpretation
Rogosa SL Broth, is known as RMW Broth, it is a modification of media formulated by Rogosa, Mitchell and Wiseman (1,2). This media is used for isolation, enumeration and identification of Lactobacilli from foodstuffs and clinical specimens (3,4). Accompanying bacterial flora is suppressed due to the low pH of the medium and also because of the high sodium acetate concentration. Rogosa SL HiVeg™ Broth is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.
HiVeg™ hydrolysate No. 1, yeast extract provide nitrogenous compounds, sulphur, trace elements and vitamin B complex, essential for growth of Lactobacilli. Dextrose, Arabinose, Saccharose are the fermentable carbohydrates. Polysorbate 80 is the source of fatty acids. Ammonium citrate and sodium acetate inhibit moulds, Streptococci and many other organisms. Potassium dihydrogen phosphate provides buffering capability. Magnesium sulphate, manganese sulphate and ferrous sulphate are sources of inorganic ions. Low pH of the medium and addition of acetic acid makes the medium selective for Lactobacilli inhibiting other bacterial flora (3).
It is recommended that the plates should be incubated at 30°C for 5 days or at 37°C for 3 days in an atmosphere of 95% hydrogen and 5% carbon dioxide (5). High acetate concentration and acidic pH suppress many strains of other lactic acid bacteria. The salt in the formulation makes the medium unsuitable for isolation of dairy lactobacilli e.g. L. lactis, L. bulgaricus and L. helveticus (3,5).
Type of specimen
Please add specimens
Specimen Collection and Handling:
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- It is recommended that the plates should be incubated at 30°C for 5 days or at 37°C for 3 days in an atmosphere of 95% hydrogen and 5% carbon dioxide (5). If this is not possible, overlay the inoculated plates with a second layer of the agar before incubation.
- High acetate concentration and acidic pH suppress many strains of other lactic acid bacteria.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous soft lumps which can be easily broken down to powder form.
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent solution in tubes
Reaction
Reaction of 5.97% w/v aqueous solution after addition of glacial acetic acid at 25°C pH : 5.4±0.2
pH
5.20-5.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 40-48 hours, in presence of 5% Carbon dioxide (CO2) and 95%H2.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Lactobacillus casei ATCC 9595 | 50-100 | good-luxuriant |
| Lactobacillus fermentum ATCC 9338 | 50-100 | good-luxuriant |
| Lactobacillus leichmanni ATCC 4797 | 50-100 | good-luxuriant |
| Lactobacillus plantarum ATCC 8014 | 50-100 | good - luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store dehydrated and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (6,7).
| Product Name | Rogosa SL HiVeg™ Broth |
|---|---|
| SKU | MV407 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Downes F. P. and Ito K., (Eds.), Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., AmericanPublic Health Association, Washington, D.C. 2.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification- Maintenance of Medical Bacteria, Vol. I, Williamsand Wilkins, Baltimore. Md. 3.Rogosa M., Mitchell J. A. and Wiseman R. F, 1951, J. Bacteriol., 62, 132-133. 4.Rogosa M., Mitchell J. A. and Wiseman R. F., 1951, J. Dental Res. 30:682. 5.Sharpe M. L. (Ed.), 1960, Lab-Practice, 9(4): 223. |
| Customized Product Available | No |
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