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HI Broth, HiVeg™ (Heart Infusion Broth, HiVeg™)

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MV170

For the isolation and cultivation of a wide variety of fastidious organisms.

Description

Intended Use

Recommended cultivation of a wide variety of fastidious organisms.

Composition

Ingredients	g/L
HiVeg™ infusion	500.000
HiVeg™ hydrolysate No. 1	10.000
Sodium chloride	5.000
Final pH (at 25°C)	7.4±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 25.0 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired 5% v/v sterile defibrinated blood may be added. Mix well and aseptically pour into sterile tubes or flasks as desired.

Principle And Interpretation

Fastidious organisms having exacting nutritional requirement could be cultivated on infusion media, as demonstrated by Huntoon (1). A liquid medium containing an infusion of meat was one of the first media used for the cultivation of bacteria. These infusion media need not be further supplemented by the addition of supplements for cultivation of fastidious bacteria (2). Heart Infusion Broth, containing infusion from beef heart is used for the isolation and cultivation of a wide variety of fastidious organisms (3). Heart infusion media can also be used for the cultivation of Vibrio species (2,4). Heart Infusion Broth can also be supplemented with glucose, horse serum and antibiotics for the cultivation a wide variety of organisms (3). Heart Infusion Broth can be used as a base to study carbohydrate fermentation. This medium was used for isolation and enumeration of haemolytic Streptococci in milk (5).

HI Broth, HiVeg™ is prepared by using vegetable peptones in place of animal based peptones which make the media free of BSE/TSE risks.

HiVeg™ hydrolysate No. 1 and HiVeg™ infusion provide nutritional requirements for the pathogenic bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.

Type of specimen

Food and dairy samples - Milk, meat samples

Specimen Collection and Handling

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (4,6,7). After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations :

Further biochemical and serological tests must be carried out for further identification.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Cream to yellow homogeneous free flowing powder

Colour and Clarity of prepared medium
Basal medium: Light yellow coloured, clear to slightly opalescent solution. After addition of 5% sterile defibrinated blood: Cherry red coloured, opaque solution in tubes

Reaction
Reaction of 2.5% w/v aqueous solution at 25°C. pH: 7.4±0.2

pH
7.20-7.60

Cultural Response
Cultural characteristics observed with added 5%w/v sterile defibrinated blood, after an incubation at 35-37°C for 18-48 hours.

Organism	Inoculum (CFU)	Growth
Escherichia coli ATCC 25922 (00013*)	50-100	good-luxuriant
Neisseria meningitidis ATCC 13090	50-100	good-luxuriant
Streptococcus pneumoniae ATCC 6303	50-100	good-luxuriant
Streptococcus pyogenes ATCC 19615	50-100	good-luxuriant
Staphylococcus aureus subsp. aureus ATCC 25923 (00034*)	50-100	good-luxuriant

Key: *Corresponding WDCM numbers.

Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).

Product Information

More Information
Product Name	HI Broth, HiVeg™ (Heart Infusion Broth, HiVeg™)
SKU	MV170
Product Type	HiVeg™
Physical Form	Powder
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1.Huntoon F. M., 1918, J. Inf. Dis., 23:169. 2.FDA Bacteriological Analytical Manual, 8th Ed., AOAC International,Gaithersburg, MD. 3.Atlas R. M., 2004, Handbook of Microbiological Media, 3rd Ed., CRC Press. 4.Downes F. P. and ItoK., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association Washington, D.C. 5.Diagnostic Procedures and Reagents, 1950, 3rd Edition, 13
Customized Product Available	No