Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Columbia Broth
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$69.30
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SKU:
LQ006
This medium is recommended for cultivation of fastidious organisms from blood. It contains Columbia broth which support luxurious growth of the organisms. The medium is buffered with tris buffer. The addition of salts was found to be beneficial for the recovery of organisms. L-Cysteine HCL is the reducing agent. Magnesium & iron are added to facilitate organism growth. It contains 20 ml for paediatric use.
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Intended Use
Recommended for cultivation of fastidious organisms from blood. Sterile, in glass bottles.
Composition**
| Ingredients | g/L |
|---|---|
| Peptone special | 10.000 |
| Biopeptone | 10.000 |
| HI powder # | 3.000 |
| L-Cysteine hydrochloride | 0.100 |
| Dextrose (Glucose) | 2.500 |
| Sodium chloride | 5.000 |
| Magnesium sulphate | 0.100 |
| Ferrous sulphate | 0.020 |
| Sodium carbonate | 0.600 |
| Tris (hydroxymethyl) aminomethane | 0.830 |
| Tris (hydroxymethyl) aminomethane hydrochloride | 2.860 |
| Final pH (at 25°C) | 7.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Heart infusion powder
Directions
Label the ready to use blood culture bottle. Remove the Aluminium foil cap. Disinfect the part of the rubber stopper which is now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35-37°C for 18-48 hours. and further for seven days.
Principle And Interpretation
Morello and Ellner in 1969 devised a liquid medium for the recovery of microorganisms from blood cultures (1). This medium was devised from Columbia Blood Agar Base previously formulated by Ellner et al (2). While studying they found that Columbia Broth was superior to a commonly used general-purpose broth for faster growth of Staphylococcus aureus, Escherichia coli, viridans Streptococci and Enterococcus groups.
In the formulation the increased concentration of cystine is provided for improved recovery of both aerobic and anaerobic microorganisms from blood specimens. It is an excellent blood culture medium (3). It differs from the agar base in that the cornstarch is omitted to reduce opalescence (4) and salts have been included. Medium contains peptone special, biopeptone and HI powder to support luxurious growth of the organisms. Dextrose is added as a carbon and energy source. The medium is buffered with tris buffer. The addition of salts was found to be beneficial for the recovery of organisms. L-Cysteine HCL is the reducing agent. Magnesium & iron are added to facilitate organism growth. Growth in tubes is indicated by presence of turbidity compared to an uninoculated control. If growth appears, cultures should be subcultured onto appropriate media.
Type of specimen
Clinical samples : Blood
Specimen Collection and Handling :
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Recommended for Paediatric use only.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile clear Columbia Broth in glass bottle.
Colour
Light amber coloured clear solution
Quantity of Medium
20ml of medium in glass bottle. (Volume of blood for paediatrics use - 1 to 3 ml)
Reaction
7.30-7.70
Sterility test
Passes release criteria
Cultural response
Cultural characteristics was observed after incubation at 35-37°C for 18-48 hours.
| Organism | Inoculum (CFU) | Growth | Growth under anaerobic conditions |
|---|---|---|---|
| Streptococcus mitis ATCC 9811 | 50-100 | luxuriant | - |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant | - |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant | - |
| Clostridium perfringens ATCC 12924 | 50-100 | - | luxuriant |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
Key: (*) Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Columbia Broth |
|---|---|
| SKU | LQ006 |
| Customized Product Available | No |
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