Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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BHI Broth
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$37.40
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Availability: In stock
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SKU:
LQ077
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Intended Use
Sterile, ready prepared 5 ml medium for propagation of pathogenic cocci and other fastidious organisms associated with blood culture work and allied pathological investigations.
Composition**
| Ingredients | g/L |
|---|---|
| HM infusion powder # | 12.500 |
| BHI powder | 5.000 |
| Proteose peptone | 10.000 |
| Dextrose (Glucose) | 2.000 |
| Sodium chloride | 5.000 |
| Disodium hydrogen phosphate | 2.500 |
Final pH (at 25°C) 7.4±0.2
Directions
- This product is available in multiple pack sizes of 25 and 50 bottles containing 5ml of sterile medium in each bottle.
- The bottles when supplied are intact. Ensure that all bottles are in upright position and there is no leakage or any manufacturing defect or contamination.
- User may remove the desired number of bottles from the box as per their requirement.
- It should be handled by trained person wearing appropriate personal protective equipment (PPE) and sterile gloves.
- Place the bottles on sterile surfaces such as laminar air flow or sterile working bench.
- Label them accordingly.
- Disinfect the outer surface of cap or closures with suitable disinfectant example 70% IPA.
- Observe aseptic techniques and standard microbiological methods while processing and inoculation of samples or cultures into this media.
- On completion of inoculation tighten the cap and close it carefully.
- Incubate at specified temperature and time or as desired.
- Follow good lab practices for procedures and disposal.
Principle And Interpretation
BHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth (1). BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds (2,3,4). This medium is nutritious and well buffered to support the growth of wide variety of organisms (3,5,6).
Proteose peptone, HM infusion powder and BHI powder serve as sources of carbon, nitrogen, essential growth factors, amino acids and vitamins. Dextrose (Glucose) serves as a source of energy. Disodium phosphate helps in maintaining the buffering action of the medium whereas sodium chloride maintains the osmotic equilibrium of the medium.
Type of specimen
Clinical samples: Blood and other pathological samples; Food and dairy samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (7,8). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (9,4,10). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- As organisms differ in their nutritional requirements, some fastidious organisms may be inhibited or may show poor growth.
- Biochemical characterization is carried out from pure isolates for complete identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile clear BHI broth in glass bottles.
Colour
Light to medium amber coloured medium
Quantity of medium
5ml of medium in bottles
Sterility Check
Passes release criteria
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after incubation at 35 - 37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Neisseria meningitidis ATCC 13090 | 50-100 | good-luxuriant |
| Streptococcus pneumoniae ATCC 6303 | 50-100 | good-luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | good-luxuriant |
| Enterococcus faecalis ATCC 29212 (00087*) | 50-100 | good-luxuriant |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | BHI Broth |
|---|---|
| SKU | LQ077 |
| Customized Product Available | No |
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