Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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HiCrome™ Enterobacter sakazakii HiVeg™ Agar, Modified (HiCrome™ Cronobacter sakazakii HiVeg™ Agar, Modified)
As low as
$75.90
Availability: Out of stock
Availability: In stock
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SKU:
MV1641
Recommended for the isolation and identification of Cronobacter sakazakii from milk and milk products.
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Intended Use
Recommended for the isolation and identification of *Cronobacter sakazakii from milk and milk products.
Key: *: Formerly known as Enterobacter sakazakii
Composition
| Ingredients | g/L |
|---|---|
| HiVeg™ hydrolysate | 7.000 |
| Yeast extract | 3.000 |
| Sodium chloride | 5.000 |
| Synthetic detergent No. III | 0.600 |
| Chromogenic substrate | 0.150 |
| Crystal violet | 0.002 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.75 grams in 1000 ml purified / distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and pour into sterile Petri plates.
Principle And Interpretation
Enterobacter species are widely distributed in nature occurring in fresh water, soil, sewage, plants, vegetables, animal and human faeces. *Cronobacter sakazakii has been closely associated with neonatal meningitis and sepsis (1). HiCrome™ Enterobacter sakazakii Agar, Modified is recommended by ISO Committee for the isolation and identification of C. sakazakii (2). HiCrome™ Enterobacter sakazakii HiVeg™ Agar, Modified is prepared by completely replacing animal based peptones with vegetable peptones to avoid BSE/TSE risks associated with animal peptones. The chromogenic substrate is cleaved specifically (3) by *C. sakazakii resulting in the formation of blue green colonies. Other organisms, which do not cleave this substrate, produce colorless to slightly violet coloured colonies. HiVeg™ hydrolysate and yeast extract provide the essential growth nutrients along with nitrogenous and carbonaceous compounds. Sodium chloride helps in maintaining the osmotic equilibrium of the medium. Synthetic detergent No. III and crystal violet inhibits the accompanying gram-positive flora.
Key: *: Formerly known as Enterobacter sakazakii
Type of specimen
Food and Dairy samples; Water and wastewater samples
Specimen Collection and Handling
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,4,5). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards (6). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Some species may show poor growth due to nutritional variations.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light purple coloured, clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 3.07% w/v aqueous solution at 25°C. pH: 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 44±1°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Colour of Colony |
|---|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | good-luxuriant | >=50% | colourless with blue centre. |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | 0% | |
| Staphylococcus aureus ATCC 25923(00034*) | >=104 | inhibited | 0% | |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | good-luxuriant | >=50% | colourless |
| ## Cronobacter sakazakii ATCC 12868 | 50-100 | good-luxuriant | >=50% | blue-green |
Key: *Corresponding WDCM numbers. # - Formerly known as Enterobacter aerogenes ##-Formerly known as Enterobacter sakazakii
Storage and Shelf Life
Store between 15-25°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (7,8).
| Product Name | HiCrome™ Enterobacter sakazakii HiVeg™ Agar, Modified (HiCrome™ Cronobacter sakazakii HiVeg™ Agar, Modified) |
|---|---|
| SKU | MV1641 |
| Product Type | HiVeg™, HiCrome™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Muytjens H. L., Zanen H. C., Sonderkamp H. J. et al, J. Clin Microbiol 18:115-120, 1983. |
| Customized Product Available | No |
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