Recommended for detection and enumeration of Enterococci by membrane filtration. Enterococci and Group D Streptococci exhibit esculin hydrolysis. Esculin is hydrolysed to esculetin and dextrose, which reacts with ferric citrate producing brownish black precipitate. Sodium azide renders selectivity to the medium. Sterile pad is supplied in sterile disposable bag . Membrane filter is not supplied .