Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Fraser Secondary Enrichment HiCynth™ Broth Base
As low as
$75.90
Availability: Out of stock
Availability: In stock
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SKU:
MCD1083
For the isolation, cultivation and enrichment of Listeria monocytogenes from food and environmental specimens.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.1* | 15.000 |
| HiCynth™ Peptone No.5* | 5.000 |
| Sodium chloride | 20.000 |
| Lithium chloride | 3.000 |
| Disodium phosphate | 12.000 |
| Monopotassium phosphate | 1.350 |
| Esculin | 1.000 |
| Ferric ammonium citrate | 0.500 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined Peptones
Directions
Suspend 57.85 grams in 990 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Enrichment Supplement (FD065) or one vial of Fraser Selective Supplement (FD125). Mix thoroughly and dispense into sterile tubes or flasks as desired.
Principle And Interpretation
Fraser Secondary Enrichment Broth is a modification of United States Department of Agriculture-Food Safety Inspection Service (USDA-FSIS) UVM Secondary Enrichment Broth. It is based on the formulation of Fraser and Sperber (1) and found to be remarkably accurate in detecting Listeria species in food and environmental samples (2). Fraser Secondary Enrichment Broth is recommended by APHA (3). Fraser Secondary Enrichment Broth Base is formulated so as to provide optimum conditions for the growth of Listeria Fraser Secondary Enrichment HiCynth™ Broth Base is the modification of the same by replacing animal peptones with chemically defined peptones to avoid BSE/TSE risk.
HiCynth™ Peptone No.1 and HiCynth™ Peptone No.5 make the media highly nutritive by providing essential nutrients including carbonaceous and nitrogenous substances, long chain amino acids, vitamins and other essential growth nutrients. Phosphates maintain the buffering capacity of the medium. All Listeria species exhibit ẞ-glucosidase activity which is evident by the blackening of the media. Listeria species hydrolyze esculin (substituted glucoside) to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate, resulting in the formation of 6-7 dihydroxycoumarin, a black brown complex. Ferric ammonium citrate also enhances the growth of L.monocytogenes (4). The high salt tolerance (of sodium chloride) of Listeria is used as means to inhibit the growth of Enterococci. Lithium chloride is also used to inhibit Enterococci, which also possess the ability to hydrolyze esculin. Growth of accompanying bacteria is largely inhibited by the addition of Nalidixic acid and Acriflavin hydrochloride (FD).
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Basal medium : Yellow coloured, clear solution with slight precipitate. After addition of FD065 or FD125: Fluorescent yellow coloured, clear solution with slight precipitate forms in tubes.
Reaction
Reaction of 5.78% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH
7.00-7.40
Cultural Response
Cultural characteristics observed with added Fraser enrichment supplement (FD065) or Fraser Selective Supplement (FD125) after an incubation at 35-37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Esculin hydrolysis |
|---|---|---|---|
| Escherichia coli ATCC 25922 | >=10³ | inhibited | |
| Enterococcus faecalis ATCC 29212 | >=10³ | inhibited | |
| Listeria monocytogenes ATCC 19111 | 50-100 | good-luxuriant | positive reaction, blackening of medium |
| Listeria monocytogenes ATCC 19112 | 50-100 | good-luxuriant | positive reaction, blackening of medium |
| Listeria monocytogenes ATCC 19117 | 50-100 | good-luxuriant | positive reaction, blackening of medium |
| Listeria monocytogenes ATCC 19118 | 50-100 | good-luxuriant | positive reaction, blackening of medium |
| Staphylococcus aureus ATCC 25923 | >=10³ | inhibited |
Storage and Shelf Life
Store below 30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label. Product performance is best if used within stated expiry period.
| Product Name | Fraser Secondary Enrichment HiCynth™ Broth Base |
|---|---|
| SKU | MCD1083 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.Fraser J.A. and Sperber W.H., 1988, Food Protect., 51(10):762.2.McClain D. and Lee W.H., 1988, J. Assoc. Off. Anal. Chem., 71(3):660.3.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.4.Cowart R. E. and Foster B. G., 1985, J. Infect. Dis.; 151:172. |
| Customized Product Available | No |
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