Listeria Enrichment Medium Base (UVM) is recommended for selective isolation and cultivation of Listeria monocytogenes from clinical specimens. The medium was originally formulated by Donnelly and Baigent. It was later modified by decreasing the nalidixic acid concentration in the selective supplements and subsequently increasing the acriflavin concentration. University of Vermont Modification Medium (UVM) used a two-step selective enrichment medium resulting in a higher isolation rate of L.monocytogenes from meat products within 3-4 days. It is recommended as a primary enrichment broth for recovery of heat-injured Listeria.