Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Potato Dextrose HiCynth™ Agar
As low as
$48.40
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
MCD096
Recommended for the isolation and enumeration of yeasts and moulds from dairy and other food products, PDA, Chemically defined
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Composition
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.2* | 4.000 |
| Dextrose | 20.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 5.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptone
Directions
Suspend 39 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates. In specific work, when pH 3.5 is required, acidify the medium with sterile 10% tartaric acid. The amount of acid required for 100 ml. of sterile, cooled medium is approximately 1 ml. Do not heat the medium after addition of the acid.
Principle And Interpretation
Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of typical varieties of dermatophytes on the basis of pigment production (4). It is also recommended by USP (5), BP (6) ,EP (7) and JP (8) for growth of fungi. Potato Dextrose HiCynth™ Agar is the modified using chemiclly defined peptone free from vegetable peptones.
HiCynth™ Peptone No.2 and dextrose promote luxuriant fungal growth by providing nitrogen and carbon source, long chain amino acids, vitamins and other growth factors. Adjusting the pH of the medium by tartaric acid to 3.5, inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyse the agar which can render the agar unable to solidify.
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Gelling: Firm, comparable with 1.5% Agar gel
Colour and clarity of prepared medium: Light amber coloured clear to slightly opalescent gel forms in Petri plates
Reaction: pH of 3.9% w/v aqueous solution at 25°C. pH: 5.6±0.2
pH: 5.40-5.80
Cultural Response
MCD096: Cultural characteristics observed after incubation at 20-25 °C for 2-7 days. Recovery rate is considered as 100% for fungus growth on Sabouraud Dextrose Agar.
| Organism | Inoculum (CFU) | Growth | Recovery |
|---|---|---|---|
| Candida albicans ATCC 10231 | 50-100 | luxuriant | >=70% |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | >=70% |
| Saccharomyces cerevisiae ATCC 9763 | luxuriant | >=70% | |
| Rhodotorula mucilaginosa DSM 70403 | luxuriant | ||
| Geotrichum candidum DSM 1240 | good- luxuriant | ||
| Penicillium communae ATCC 10248 | fair -good | ||
| Trichophyton ajelloi ATCC 28454 | fair-good |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Potato Dextrose HiCynth™ Agar |
|---|---|
| SKU | MCD096 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. 2.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC. 3.Wehr H. M. and Frank J. H., 2004, Standard Methods for the Microbiological Examination of Dairy Products, 17th Ed.,APHA Inc., Washington, D.C. 4.MacFaddin J. F., 1985, Media for the Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol.1, Williamsand Wilkins, Baltimore5.The United States Pharmacopoeia, 2016, The United States Pharmacopoeial Convention. Rockville, MD. 6.British Pharmacopoeia, 2016, The Stationery office British Pharmacopoeia7.European Pharmacopoeia, 2014, European Dept. for the quality of Medicines. 8.Japanese Pharmacopoeia, 2008. |
| Customized Product Available | No |
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