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Sabouraud Dextrose HiVeg™ Broth, Granulated (Sabouraud Liquid HiVeg™ Medium, Granulated)

Name: Sabouraud Dextrose HiVeg™ Broth, Granulated (Sabouraud Liquid HiVeg™ Medium, Granulated)
SKU: GMV033
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GMV033

for cultivation of yeasts, moulds and aciduric microorganisms.

Description

Sabouraud Dextrose HiVeg™ Broth, granulated is used for cultivation of yeasts, moulds and aciduric microorganisms.

Composition

Ingredients	Gms/Litre
Dextrose	20.000
HiVeg special peptone	10.000
Final pH (at 25°C)	5.6±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 30 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense in tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Principle And Interpretation

Sabouraud Dextrose HiVeg Broth is prepared by using HiVeg special peptone in place of Peptone special which is free of BSE/ TSE risks. It is the modification of Dextrose Agar described by Sabouraud (1). The medium is recommended by APHA for the cultivation of fungi, particularly associated with skin infections(2). Sabouraud dextrose media are peptone media supplemented with dextrose to support the growth of fungi. HiVeg special peptone provides nitrogen, vitamins, minerals, amino acids and growth factors essential for the growth of fungi. Dextrose acts as the energy source. The low pH favours fungal growth and inhibits contaminating bacteria from clinical specimens(3). The acid reaction of the final medium is inhibitory to a large number of bacteria making it particularly useful for cultivating fungi and aciduric microorganisms. For isolation of fungi from contaminated specimens, a selective medium should be inoculated simultaneously. Incubate cultures for 4 to 6 weeks before reporting as negative.

Quality Control

Appearance: Cream to yellow coloured granular medium

Colour and Clarity of prepared medium: Light amber Clear solution

Reaction: Reaction of 3.0% w/v aqueous solution at 25°C. pH: 5.6±0.2

pH: 5.40-5.80

Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 3 days.

Cultural Response

Organism	Inoculum (CFU)	Growth
*Aspergillus brasiliensis ATCC 16404	50-100	Luxuriant
Candida albicans ATCC 10231	50-100	Luxuriant
Candida albicans ATCC 2091	50-100	luxuriant
Escherichia coli ATCC 25922	50-100	good-luxuriant (inhibited on media with lower pH)
Escherichia coli NCTC 9002	50-100	Luxuriant
Escherichia coli ATCC 8739	50-100	good-luxuriant (inhibited on media with lower pH)
Saccharomyces cerevisiae ATCC 2601	50-100	good-luxuriant
Saccharomyces cerevisiae ATCC 9763	50-100	good-luxuriant
Lactobacillus casei ATCC 334	50-100	good-luxuriant (inhibited on media with lower pH)

* Formerly known as Aspergillus niger

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Product Information

More Information
Product Name	Sabouraud Dextrose HiVeg™ Broth, Granulated (Sabouraud Liquid HiVeg™ Medium, Granulated)
SKU	GMV033
Product Type	Granulated
Physical Form	Granular
Origin	Animal Free (Veg)
Packaging type	HDPE
References	1. Carlier G. I. M., 1984, Brit. J. Derm. Syph., 60:61 2.Sabouraud R., 1892, Ann. Dermatol. Syphil. 3 : 1061. 3.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C. 4.Sabouraud R., Les Teignes, Paris: Masson et Cie, 1910, p 553 5.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,8th Ed., American Society for Microbiology, Washington, D.C. 6.American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., WashingtonD.C. 7.Isenberg, H.D. Clinical Microbiology Procedures Handb0ook. 2nd Edition. 8.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1.
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