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Soyabean Casein Digest HiCynth™ Medium (Tryptone Soya HiCynth™ Broth)
As low as
$28.60
Availability: Out of stock
Availability: In stock
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SKU:
MCD011
Recommended as a general purpose medium used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.
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Soyabean Casein Digest HiCynth™ Medium is a general purpose medium used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| HiCynth™ Peptone No.3* | 17.000 |
| HiCynth™ Peptone No.5* | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
*Chemically defined peptones
Directions
Suspend 30 grams in 1000 ml purified/ distilled water. Heat if necessary to dissolve the medium completely. Mix well and dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Note: If any fibres are observed in the solution, it is recommended to filter the solution through a 0.22 micron filter to eliminate the possibility of presence of fibres.
Principle And Interpretation
Soyabean Casein Digest Medium is recommended by various pharmacopoeias as a sterility testing and as a microbial limit testing medium (1, 2, 3). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (4). Soyabean Casein Digest HiCynth™ Medium is the modification of the same using chemically defined peptone free from animal and vegetable peptones to avoid BSE/TSE risks associated with animal peptones.
HiCynth™ peptone No. 3 and HiCynth™ peptone No. 5 provides nitrogen and carbon source, amino acids, long chain peptides and vitamins for the growth of microorganisms. Dextrose and dibasic potassium phosphate serve as the carbohydrate source and the buffer, in the medium. Sodium chloride maintains the osmotic balance of the medium. respectively
Quality Control
Appearance: Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium: Light yellow coloured clear solution without any precipitate.
Reaction: pH of 3.0% w/v aqueous solution at 25°C. pH: 7.3±0.2
pH: 7.10-7.50
Stability test: Light yellow coloured clear solution without any precipitation or sedimentation at room temperature for 7 days
Growth promoting properties
Clearly visible growth of microorganism comparable to that previously obtained with previously tested and approved lot of medium occurs at the specified temperature for not more than the shortest period of time specified inoculating not more than 100 cfu (at 30-35°C for 18-24 hours for bacteria)
Growth promotion is carried out as per USP/EP/BP/JP.
| Organism | Inoculum (CFU) | Growth | Incubation temperature | Incubation period |
|---|---|---|---|---|
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 30-35 °C | 18-24 hrs |
Sterility Testing + Validation
The medium is tested with suitable strains of microrganisms inoculating <=100cfu and incubating at 20-25°C for not more than 3 days in case of bacteria and not more than 5 days in case of fungi.
| Organism | Inoculum (CFU) | Growth | Incubation temperature | Incubation period |
|---|---|---|---|---|
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Staphylococcus aureus ATCC 25923 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 20-25 °C | <=3 d |
| Candida albicans ATCC 10231 | 50-100 | luxuriant | 20-25 °C | <=5 d |
| Candida albicans ATCC 2091 | 50-100 | luxuriant | 20-25 °C | <=5 d |
| *Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | 20-25 °C | <=5 d |
Storage and Shelf Life
Store below 30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Soyabean Casein Digest HiCynth™ Medium (Tryptone Soya HiCynth™ Broth) |
|---|---|
| SKU | MCD011 |
| Product Type | HiCynth™ |
| Physical Form | Powder |
| Origin | Chemically defined (HiCynth™) |
| Packaging type | HDPE |
| References | 1. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams& Wilkins, Baltimore, M.d. 2.The United States Pharmacopeia, 2018, USP 41/NF36, The United States Pharmacopeial Convention, Rockville, MD. 3.Indian Pharmacopeia, 2018, Govt. of India, Ministry of Health and Family Welfare, New Delhi, India. 4.Forbes B. A., Sahm D. F. and Weissfeld A. S., 1998, Bailey & Scotts Diagnostic Microbiology, 10th Ed., Mosby, Inc. St.Louis, Mo. 5.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition. 6.Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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