Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
Your enquiry has been submitted
Something problem with the server. Try again.
TSB - Tryptone Soya Broth (Soyabean Casein Digest Medium)
SKU:
LQ009
Availability: In stock
Only %1 left
As low as
$49.00
Intended Use
A qualitative test for detection of microorganisms in blood. Sterile, in glass bottles.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 17.000 |
| Soya peptone | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose (Glucose) | 2.500 |
| Dipotassium hydrogen phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use blood culture bottle. Do not unscrew cap. remove the top of the screw cap. Disinfect the part of the rubber stopper which is now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35±2°C for 18-24 hours and further for seven days.
Principle And Interpretation
Soyabean Casein Digest Medium is recommended by various pharmacopoeias as a sterility testing and as a microbial limit testing medium (1,2). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (3,4). Bacteremia is a serious and often life-threatening clinical condition. An important diagnostic tool for this condition is to analyze a blood specimen for the growth of bacteria on selected growth media.
The combination of tryptone and soya peptone makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose (Glucose) and dipotassium hydrogen phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium.
Type of specimen
Clinical sample: Blood
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature..
Quality Control
Appearance
Sterile clear Tryptone Soya Broth in glass bottle.
Colour
Light yellow coloured clear solution
Quantity of Medium
20 ml of medium in glass bottle, (Volume of blood for paediatrics use - 1 to 3 ml )
pH
7.10-7.50
Sterility Check
Passes release criteria.
Cultural response
Cultural characteristics observed after an incubation at 35-37°C for 18-48hrs
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| Streptococcus pyogenes ATCC 19615 | 50-100 | luxuriant |
| Candida albicans ATCC 10231 (00054*) | 50-100 | luxuriant |
| Neisseria meningitidis ATCC 13090 | 50-100 | luxuriant |
| #Phocaeicola vulgatus ATCC 8482 | 50-100 | luxuriant |
Key: (*) Corresponding WDCM numbers, **Formerly known as Bacillus subtilis subsp. spizizenii
# Formerly known as Bacteroides vulgatus
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
| Product Name | TSB - Tryptone Soya Broth (Soyabean Casein Digest Medium) |
|---|---|
| SKU | LQ009 |
Write Your Own Review
How long does it take to get results from sterility testing with TSB?
USP <71> requires minimum 14 days incubation for both TSB and FTM. However, most contamination is detected within 3-7 days. Daily visual inspection for turbidity, color change, or visible growth. Many labs examine: Days 1-5 daily, Days 6-14 every 2-3 days. Positive results must be confirmed by subculture and identification. Some regulatory agencies accept 7-day incubation for specific validated products, but 14 days is standard. Rapid methods (ATP bioluminescence, PCR) provide faster results but aren't USP <71> compliant.
What is the composition of Tryptic Soy Broth?
TSB contains: Trypticase peptone (17 g/L) - provides amino acids and peptides; Soya peptone (3 g/L) - provides carbohydrates and vitamins; Sodium chloride (5 g/L) - maintains osmotic balance; Dipotassium hydrogen phosphate (2.5 g/L) - buffering; Dextrose (2.5 g/L) - energy source. Final pH 7.3±0.2. This nutrient-rich formulation supports rapid growth of diverse microorganisms. No inhibitory substances, making it suitable for stressed or injured cells.
Can I use TSB for bacterial culture outside of sterility testing?
Yes, TSB is an excellent general-purpose enrichment broth for cultivation of aerobic and facultative anaerobic bacteria. Applications include: (1) Pre-enrichment in food microbiology, (2) Revival of stressed organisms, (3) Biomass production, (4) Antibiotic susceptibility test inoculum preparation, (5) Sub-culturing from agar plates, (6) Blood culture bottle enrichment. Supports growth of fastidious organisms including Streptococcus, Neisseria, Listeria, and Brucella species.
How many TSB tubes are required per sterility test?
Per USP <71>, use at least 2 TSB tubes per sterility test, plus 2 FTM tubes (4 tubes total minimum). For membrane filtration of large volume products: test each filter in separate medium containers. For products in containers >40mL: test contents from each container in separate medium. For validation studies: include positive controls (known viable organisms) and negative controls (uninoculated media). Number of tubes increases with product batch size and regulatory requirements.
What organisms are detected with TSB that might be missed by FTM?
TSB at 20-25°C preferentially recovers: (1) Candida species (common yeast contaminants), (2) Aspergillus species (common mold contaminants), (3) Other filamentous fungi, (4) Psychrophilic bacteria from Water systems, (5) Environmental organisms adapted to room temperature. While FTM can support some fungi, TSB's nutrient-rich formulation and lower incubation temperature maximize fungal recovery. Some fastidious aerobic bacteria also grow better in TSB than FTM.
Why is TSB incubated at 20-25°C instead of 35-37°C?
TSB is incubated at 20-25°C to optimize recovery of fungi (yeasts and molds) and psychrophilic bacteria that may contaminate pharmaceutical products. Many fungal contaminants grow better at room temperature than at body temperature. The lower incubation temperature also reduces metabolic stress on environmental contaminants. This complements FTM incubated at 30-35°C for bacteria. Together, the two temperature ranges ensure detection of the broadest possible range of microbial contaminants.
What is Tryptic Soy Broth RTU used for?
Tryptic Soy Broth (TSB) RTU 20mL is used as the aerobic/fungal medium for USP <71> sterility testing. Incubated at 20-25°C, TSB supports growth of aerobic bacteria, yeasts, and molds. Used in combination with Fluid Thioglycollate Medium (FTM at 30-35°C) to provide comprehensive sterility testing coverage. The 20mL format is standard for most pharmaceutical sterility testing applications and accommodates samples up to 2mL volume per tube.