Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Soyabean Casein Digest Medium (Tryptone Soya Broth)
Availability: Out of stock
Availability: Out of stock
Availability: Out of stock
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SKU:
LQ508
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Sterility testing media also used as a general purpose medium for the growth of aerobes & fungi.
Composition**
| Ingredients | Gms/Litre |
|---|---|
| Pancreatic digest of casein | 17.000 |
| Papaic digest of soyabean meal | 3.000 |
| Sodium chloride | 5.000 |
| Dextrose | 2.500 |
| Dibasic potassium phosphate | 2.500 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
Principle And Interpretation
Soyabean Casein Digest Medium is recommended by various pharmacopeias as a sterility testing and as a microbial limit testing medium (1, 2, 3). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (4).
The combination of pancreatic digest of casein and papaic digest of soyabean meal makes the medium nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Dextrose and dibasic potassium phosphate serve as the carbohydrate source and the buffer, respectively in the medium. Sodium chloride maintains the osmotic balance of the medium.
Quality Control
Appearance
Sterile Tryptone Soya Broth in a glass bottle.
Colour
Light yellow coloured clear solution
Quantity of Medium
200 ml of medium in glass bottle.
pH
7.10-7.50
Growth Promotion Test
In accordance with the harmonized method of USP/EP/BP/JP.
Sterility Test
Passes release criteria.
Cultural Response
| Organism | Inoculum (CFU) | Growth | Temperature | Incubation Period |
|---|---|---|---|---|
| Growth at 30-35°C for <= 3 days | ||||
| Escherichia coli ATCC 25922 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Escherichia coli ATCC 8739 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Bacillus subtilis ATCC 6633 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Staphylococcus aureus ATCC25923 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Staphylococcus aureus ATCC 6538 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Micrococcus luteus ATCC 9341 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Streptococcus pneumoniae ATCC 6305 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Pseudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Pseudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant | 30-35°C | <= 3 days |
| Growth at 20-25°C for <= 5 days | ||||
| Candida albicans ATCC10231 | 50-100 | luxuriant | 20-25°C | <= 5 days |
| Candida albicans ATCC 2091 | 50-100 | luxuriant | 20-25°C | <= 5 days |
| Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | 20-25°C | <= 5 days |
Storage and Shelf Life
Store between 15-25°C. Use before expiry date on the label.
| Product Name | Soyabean Casein Digest Medium (Tryptone Soya Broth) |
|---|---|
| SKU | LQ508 |
| Customized Product Available | No |
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