SPS Agar, Modified is recommended for selective isolation and enumeration of Clostridium perfringens from foodstuffs. SPS (Sulphite Polymyxin Sulphadiazine) Agar was developed by Angelotti et al based on the Wilson and Blair medium and the medium described by Mossel et al for selective isolation and enumeration of Clostridium perfringens from foods. The modified SPS Agar however obviates the inclusion of Miller-Prickett tubes.
1.Angelotti R., Han H. E., Foter M. J. and Lewis K. H., 1962, Appl. Microbiol., 10:193.2.Mossel D. A. A., De Bruit A. S., Van Dipen H. M. J., Vendring3.C. M. A. and Zoutewelle G., 1956, J. Appl. Microbiol., 19:142.4.Mossel R. S., 1959, J. Sci. Food Agric., 19:662.5.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,APHA, Washington, D.C.6.MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williamsand Wilkins, Baltimore.