Rippey-Cabelli Agar Base

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M859
Recommended for differential and selective isolation of Aeromonas hydrophila from water samples using membrane filter technique.


Rippey-Cabelli Agar Base is recommended for differential and selective isolation of Aeromonas hydrophila from water samples using membrane filter technique. This medium formulated by Rippey and Cabelli is a differential medium as it depends on the ability of organisms to ferment trehalose and selective due to the incorporation of selective agents. Ampicillin, sodium deoxycholate and ethanol are the selective agents inhibiting gram-positive bacteria, coliforms, Shigella species, Proteus mirabilis and Actinomyces. Ethanol inhibits overgrowth of Klebsiella species on the filter. Most of the Enterobacteriaceae ferment trehalose, therefore it is difficult to distinguish Aeromonas from Enterobacteriaceae. The medium gives higher specificity and sensitivity when pure cultures are used. Membrane filters through which water samples have been filtered are aseptically placed on Rippey-Cabelli Agar Base plates and incubated at 35-37°C for 24 hours. Aeromonas ferments trehalose and forms yellow colonies.
More Information
Product Name Rippey-Cabelli Agar Base
SKU M859
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1. Eaton A. D., Clesceri L. S. and Greenberg A. E., (Eds.), 1995, Standard Methods for the Examination of Water andWastewater, 19th Ed., American Public Health Association, Washington, D.C.2.Austin B., Altwegg M., Gosling P. and Joseph S. W., (Eds.), 1996, The Genus Aeromonas, John Wiley and Sons, Chichester ,U.K.3.Rippey S. R. and Cabelli V. J., 1979, Appl. Environ. Microbiol., 38(1): 108.4.MacFaddin J. F., 1985, Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria, Vol. I Williamsand Wilkins, Baltimore.5.Roland, F. P., 1977, Med. Microbiol. Immunol., 163:241.6.Von Graevenitz A. and Bucher C., 1983, J. Clin. Microbiol., 17(1):16.
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