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Buffered Peptone Water

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M614S
Recommended for pre-enrichment of injured Salmonella species from food prior to selective enrichment and isolation. It is recommended by BIS committee under the specifications IS:5887(Part III)-1999, reaffirmed 2005.

Buffered Peptone Water is used for pre-enrichment of injured Salmonella species from foods prior to selective enrichment and isolation. It is recommended by BIS committee under the specifications IS:5887(Part III)-1999.

Composition**

Ingredients Gms / Litre
Peptic digest of animal tissue 10.000
Sodium chloride 5.000
Disodium phosphate.12H2O 9.000
Monopotassium phosphate 1.500
Final pH (at 25°C) 7.0±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 20.07 grams of dehydrated medium in 1000 ml distilled water. Dispense in 50 ml amounts. Sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes.

Principle And Interpretation

Edel and Kampelmacher (1) noted that sublethal injury to Salmonellae may occur in many food preservation processes. Enriching injured cells in Lactose broth (M1003S) (pH 6.9) may be further detrimental to their recovery (2). Pre-enrichment in Buffered Peptone Water at 35°C for 18-24 hours results in repair of injured cells (3). Recently ISO committee has also recommended this pre-enrichment medium for the detection of Enterobacteriaceae (4). Present formulation is recommended by BIS as a non-selective pre-enrichment medium as well as a diluent for detection of Salmonella (5).

Inoculate the test sample in Buffered peptone water and incubate at 35 - 37°C for 16 - 20 hours. Transfer the culture to selective enrichment media, Modified Rappaport Vassiliadis Medium (M11371) and Fluid Selenite Cystine Broth (M0251). Incubate M11371 at 42°C and M0251 at 35 - 37°C for 24 hours. Subculture on selective plating media. Examine the plates for colonies of Salmonella species.

Quality Control

Appearance
Cream to yellow coloured homogeneous free flowing powder

Colour and Clarity of prepared medium
Light yellow coloured clear solution without any precipitate.

Reaction
Reaction of 2.0% w/v aqueous solution at 25°C. pH: 7.0±0.2

pH
6.80-7.20

Cultural Response

M614S: Cultural characteristics observed after an incubation at 35-37°C for 18 - 24 hours.

Organism Inoculum (CFU) Growth
Salmonella Typhimurium ATCC 14028 50-100 luxuriant
Salmonella Typhi ATCC 19430 50-100 luxuriant
Salmonella Enteritidis ATCC 13076 50-100 luxuriant

Storage and Shelf Life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

Reference

  1. Edel W. and Kampelmacher E.H., 1973, Bull. Wld. Hlth. Org., 48:167.
  2. Angelotti R., 1963, “Microbiological Quality of Foods", Academic Press, New York.
  3. Sadovski A.Y., 1977, J. Fd. Technol., 12:85.
  4. International Organization for Standardization (ISO), 1993, Draft ISO/DIS, 6579.
  5. Bureau of Indian Standards, IS: 5887 (Part 3) 1999.
More Information
Product Name Buffered Peptone Water
SKU M614S
Product Type Regular
Physical Form Powder
Origin Animal
Packaging type HDPE
References 1.Edel W. and Kampelmacher E.H., 1973, Bull. Wld. Hlth. Org., 48:167.
2.Angelotti R., 1963, “Microbiological Quality of Foods”, Academic Press, New York.
3.Sadovski A.Y., 1977, J. Fd. Technol., 12:85.
4.International Organization for Standardization (ISO), 1993, Draft ISO/DIS, 6579.
5.Bureau of Indian Standards, IS : 5887 (Part 3) 1999
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