Phosphate Buffered Saline, pH 7.2

Phosphate Buffered Saline, pH 7.2 is used for preparation of dilutions and blanks.

Composition**

Final pH ( at 25°C) : 7.2±0.2

**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 10.79 grams in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 10 minutes.

Principle And Interpretation

Phosphate Buffered Saline is used for preparing dilutions, blanks for the examination of waters, dairy products, foods, utensils and other specimens. This solution gives a pH of 7.2 and also provides potassium, sodium and phosphate ions (1) .

Quality Control

Appearance: White to cream homogeneous free flowing powder

Colour and Clarity of prepared medium: Colourless clear solution without any precipitate

Reaction: Reaction of 1.08% w/v aqueous solution at 25°C. pH : 7.2±0.2

pH: 7.00-7.40

Storage and Shelf Life

Store below 30°C and the prepared medium at 2-8°C. Use before expiry date on the label.

Reference

1. Clesseri, Greedberg and Eatton (ed). 1988. Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C.