Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Listeria Identification Broth Base (PALCAM), Granulated
As low as
$545.60
Availability: Out of stock
Availability: In stock
Only %1 left
SKU:
GM1090
For selective enrichment and identification of Listeria species.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Peptic digest of animal tissue | 23.000 |
| Yeast extract | 5.000 |
| Lithium chloride | 10.000 |
| Esculin | 0.800 |
| Ammonium ferric citrate | 0.500 |
| D-Mannitol | 5.000 |
| Soya lecithin | 1.000 |
| Polysorbate 80 | 2.000 |
| Phenol red | 0.080 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23.69 grams in 500 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile reconstituted contents of 1 vial of Listeria Selective Supplement (PALCAM) (FD061). Mix well before dispensing into sterile test tubes or flasks as desired.
Warning: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin, wash with plenty of water immediately.
Principle And Interpretation
The heightened awareness and concern surrounding the presence of Listeria monocytogenes in food has resulted in the development of many media for its isolation (3). Listeria Identification Broth also known as Polymyxin-Acriflavin-Lithium chloride-Ceftazidime-Aesculin-Mannitol (PALCAM) Broth is prepared as described by van Netten et al (1) for selective enrichment of Listeria species.
Peptic digest of animal tissue and yeast extract provide growth nutrients. High amount of lithium chloride and added selective supplement (FD061) (2) containing polymyxin B, acriflavin hydrochloride and ceftazidime inhibit accompanying microflora and allow the growth of Listeria species. Soya lecithin has similar properties as that of egg yolk; hence additional supplementation of egg yolk emulsion is not required.
After incubation at 30°C for 24-48 hours, approximately 0.1 ml of the broth is streaked on Listeria selective agars such as Listeria Identification Agar (PALCAM) (GM1064/M1064) or Listeria Oxford Agar (GM1145/M1145). The combination of mannitol and phenol red helps the detection of mannitol fermentation while esculin and ammonium ferric citrate together help in detection of esculin hydrolysis.
L. monocytogenes hydrolyses esculin resulting in the formation of black coloured medium.
L. monocytogenes does not ferment mannitol, therfore its differentiation from contaminants such as Enterococci and Staphylococci can be made as the later will ferment mannitol and produce a colour change from red to yellow. Incubation under microaerophilic conditions serves to inhibit strict aerobes such as Bacillus and Pseudomonas species. Techniques for the isolation of L. monocytogenes will depend on the material under test. It is usual for the test sample to be first inoculated into an enrichment broth to allow multiplication before isolation and identification. Depending on the types of samples used, the appropriate method and selective enrichment broth should be used.
Quality Control
Appearance: Light yellow to pink coloured granular medium
Colour and Clarity of prepared medium: Red coloured, clear solution without any precipitate
Reaction: Reaction of 4.74% w/v aqueous solution at 25°C. pH: 7.4±0.2
pH: 7.20-7.60
Cultural Response: Cultural characteristics observed with added Listeria Selective Supplement (PALCAM) (FD061), after an incubation at 30°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Colour of medium |
|---|---|---|---|
| Enterococcus faecalis ATCC 29212 | >=103 | inhibited | |
| Listeria monocytogenes ATCC 19118 | 50-100 | good | black |
| Micrococcus luteus ATCC 10240 | >=103 | inhibited | |
| Staphylococcus aureus ATCC 25923 | >=103 | inhibited |
Storage and Shelf Life
Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label.
Reference
- Van Netten P. et al, 1989, Int. J. Food Microbiol., 8:299.
- Lund A. M., 1991, J. Food Prot., 54:602.
- Farber J. M. and Peterkin P., 1991, Microbiol. Rev. 55: 476-511
| Product Name | Listeria Identification Broth Base (PALCAM), Granulated |
|---|---|
| SKU | GM1090 |
| Product Type | Granulated |
| Physical Form | Granular |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Van Netten P. et al, 1989, Int. J. Food Microbiol., 8:299. |
| Customized Product Available | No |
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