Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Enterococcus Confirmatory HiVeg media are recommended for confirming the presence of Enterococci in water supplies and other sources.
Product Profile
Vegetable based (Code MV)
MV392/MV394/MV419
HiVeg hydrolysate
Animal based (Code M)
M392/M394/M419
Casein enzymic hydrolysate
Recommended for
Confirming the presence of Enterococci in water supplies and other sources.
Reconstitution
- (MV392): 30.4 g/l
- (MV394): 80.4 g/l
- (MV419): 15.4 g/l
Quantity on preparation
- (500g): (MV392): 16.44 L
- (500g): (MV394): 6.21 L
- (500g): (MV419): 32.46 L
pH (25°C)
- (MV392) & (MV394): 8.0±0.2
- (MV419): 8.4 ± 0.2
Supplement
Penicillin
Sterilization
121°C / 15 minutes.
Storage
Dry Medium - Below 30°C, Prepared Medium 2-8°C.
Composition
| Ingredients | MV392 Grams/Litre |
MV394 Grams/Litre |
MV419 Grams/Litre |
|---|---|---|---|
| HiVeg hydrolysate | 5.00 | 5.00 | 5.00 |
| Yeast extract | 5.00 | 5.00 | 5.00 |
| Dextrose | 5.00 | 5.00 | 5.00 |
| Sodium azide | 0.40 | 0.40 | 0.40 |
| Methylene blue | 0.01 | 0.01 | — |
| Sodium chloride | — | 65.00 | — |
| Bromo thymol blue | — | — | 0.032 |
| Agar | 15.00 | — | — |
| Final pH (at 25°C) | 8.0 ± 0.2 | 8.0 ± 0.2 | 8.4 ± 0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 30.4 grams of MV392 or 80.4 grams of MV394 or 15.4 grams of MV419 in 1000 ml distilled water. Boil to dissolve the medium completely. Dispense in 100 ml quantities in tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Allow the agar tubes to cool in a slanted position and cool the broth (MV394) to room temperature and add 65 units of Penicillin to each 100 ml of broth prior to use.
Warning: Sodium azide has a tendency to form explosive metal azides with plumbing materials. It is advisable to use enough water to flush off the disposables.
Principle and Interpretation
These media are prepared by using HiVeg hydrolysate which is free of BSE/TSE associated risks. These Enterococcus media are the modification of media formulated by Sandholzer and Winter (1) for the detection of Enterococci in water supplies, swimming pools, sewage etc. The enterococci portion of the fecal Streptococcus group is a valuable indicator for determining the extent of fecal contamination of recreational surface waters (2). The Enterococcus Confirmatory HiVeg Broth has same formula as Enterococcus Confirmatory HiVeg Agar except agar, sodium chloride and Penicillin which is used to detect Enterococci from crab meat and oysters etc.
HiVeg hydrolysate, Yeast extract, Dextrose provide essential growth nutrients for Enterococci. Sodium azide inhibits gram-negative organisms. Penicillin has inhibitory effect on Staphylococci. High concentration of NaCl (6.5%) serves selective enrichment of Enterococcus in MV394 (3). The positive presumptive tests are confirmed by inoculating Enterococcus Presumptive HiVeg Broth (MV419) to Enterococcus Confirmatory slant-broth combination prepared with an Azide Agar medium (Enterococcus Confirmatory HiVeg Agar, MV392) overlaid with a Salt Azide Penicillin HiVeg Broth (Enterococcus Confirmatory HiVeg Broth, MV394). A negative catalase test is considered confirmed positive evidence of the presence of Enterococci. Single strength medium can be used for small inoculum. Production of acid and turbidity in an azide presumptive broth when incubated at 45°C is considered positive presumptive evidence for the presence of Enterococci which is confirmed by inoculating in / on Confirmatory Broth/ Agar (MV394, MV392).
Quality Control
Appearance of Powder
Yellow coloured, may have slightly greenish tinge, homogeneous, free flowing powder.
Gelling
Firm, comparable with 1.5% Agar gel of MV392.
Colour and Clarity
Yellow coloured, slightly opalescent gel of MV392 / clear solution of MV394 and MV419 forms in petri plates / tubes which acquires greenish tinge at the surface on standing.
Reaction
Reaction of 3.04% w/v of MV392 or 8.04% w/v of MV394 aqueous solution is pH 8.0 ± 0.2 at 25°C. Reaction of 1.54% w/v of MV419 aqueous solution is pH 8.4 ± 0.2 at 25°C.
Cultural Response
Cultural characteristics was observed after an incubation at 45°C for 24 hours.
| Organisms (ATCC) | Inoculum (CFU) |
Growth | Recovery | Acid |
|---|---|---|---|---|
| Enterococcus faecalis (29212) | 20-60 | luxuriant | >70% | + |
| Escherichia coli (25922) | 103 | inhibited | 0% | — |
Key: + = acid production, yellow colour
— = no acid production, no colour change.
References
- Sandholzer and Winter, 1946, Commercial Fisheries Leaflet T1a.
- Eaton A.D., Clesceie L.S. and Greebey A.E., (Eds.), 2005 Standard Methods for the Examination of Water and Wastewater, 21st Ed., APHA, Washington, D.C.
- Splittstoesser, Wright and Hucker, 1961, Appl. Micrbiol 9(4):303.
| Product Name | Enterococcus Confirmatory HiVeg™ Broth |
|---|---|
| SKU | MV394 |
| Product Type | HiVeg™ |
| Physical Form | Powder |
| Origin | Animal Free (Veg) |
| Packaging type | HDPE |
| References | 1. Sandholzer and Winter, 1946, Commercial Fisheries Leaflet T1a . 2.Eaton A. D., Clesceri L. S. and Greenberg A. E., (Ed.), 1998, Standard Methods for the Examination of Water andWastewater, 20th Ed., American Public Health Association, Washington, D.C. |
| Customized Product Available | No |
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