Enterococcus Confirmatory Broth is recommended for confirming the presence of Enterococci in water supplies and other sources. Enterococci are differentiated from other Streptococci by their ability to grow in 6.5% sodium chloride, at pH 9.6 and at 10°Cand 45°C. Production of acid and turbidity in an azide presumptive broth when incubated at 45°C is considered positive presumptive evidence for the presence of Enterococci which is confirmed by inoculating in /on Confirmatory Broth (M394).