Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate, Thane(W)-400604, MH, India
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Vancomycin Resistant Enterococci (VRE) Agar Plate
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SKU:
MP1763
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Intended Use
Recommended for selective isolation of vancomycin resistant Enterococci.
Composition**
| Ingredients | g/L |
|---|---|
| Tryptone | 20.000 |
| Yeast Extract | 5.000 |
| Sodium chloride | 5.000 |
| Sodium citrate | 1.000 |
| Aesculin | 1.000 |
| Ferric ammonium citrate | 0.500 |
| Sodium azide | 0.150 |
| Van 3 Selective Supplement (FD261) | 2 vial |
| Vancomycin | 3mg |
| MER Selective Supplement (FD262) | 2 vial |
| Meropenem | 1mg |
| Agar | 10.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Either streak, inoculate or surface spread the test inoculum (50-100 CFU) aseptically on the plate.
Principle And Interpretation
Enterococci usually occur as the normal flora in the intestines of mammals. The presence of enterococci is an indication of faecal contamination (1). The increasing development of multiple resistance towards antibiotics particularly vancomycin by enterococci is a serious threat to the world (2). Vancomycin-resistant Enterococcus (VRE) is the name given to a group of bacterial species of the genus Enterococcus that are resistant to the antibiotic vancomycin. Vancomycin resistanct Enterococci Agar is formulated as per the recommendations of Centre for Disease Control and Prevention (CDC) for the selective isolation of vancomycin resistant enterococci (3). Tryptone and yeast extract provides nitrogeneous, carbonaceous compounds and other essential growth nutrients to the medium. Sodium chloride maintains the osmotic balance. Enterococci species hydrolyze esculin to glucose and esculetin. The latter combines with ferric ions of ferric ammonium citrate to form a dark brown or black complex visualized as a zone of black precipitate around the colonies. Sodium azide inhibits most of the accompanying microflora. Van 3 Selective Supplement(FD261) helps in the selective isolation of vancomycin resistant enterococci from other enterocci. MER Selective Supplement(FD262) added to the medium helps to suppress the contaminating flora especially gram-negative bacteria.
Type of specimen
Clinical samples - faeces
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred to in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.Further biochemical and serological tests must be carried out for further identification.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Vancomycin Resistant Enterococci (VRE) Agar Plate in 90 mm disposable plates with smooth surface and absence of black particles/cracks/bubbles
Colour of medium: Light amber coloured medium
Quantity of medium: 25 ml of medium in 90 mm disposable plates.
pH: 6.80-7.20
Sterility Check: Passes release criteria
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 24-48 hours.
| Organism | Inoculum (CFU) | Growth | Recovery | Esculin Hydrolysis |
|---|---|---|---|---|
| Enterococcus faecalis ATCC 29212 (00087*) | >=10³ | inhibited | 0% | negative reaction |
| Enterococcus faecalis NCTC 12201 | 50-100 | luxuriant | >=50% | positive reaction, blackening of medium |
| Escherichia coli ATCC 25922 (00013*) | >=10³ | inhibited | 0% | |
| Salmonella Typhimurium ATCC 14028 (00031*) | >=10³ | inhibited | 0% | |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | >=10³ | inhibited | 0% | |
| Enterococcus faecium NCTC 12202 | 50-100 | luxuriant | >=50% | positive reaction, blackening of medium |
| Enterococcus faecalis ATCC 51299 (00085*) | 50-100 | luxuriant | >=50% | positive reaction, blackening of medium |
Key: *Corresponding WDCM numbers.
Storage and Shelf Life
On receipt store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Vancomycin Resistant Enterococci (VRE) Agar Plate |
|---|---|
| SKU | MP1763 |
| Customized Product Available | No |
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