HiVibri-O-Trap Kit

Principle and Interpretation

Vibrio species are gram negative bacteria. They are salt tolerant and are found in environments with higher salinity. Vibrio has a major occurrence in sea food, which can lead to food poisoning. This gives Vibrio clinical significance. Regular monitoring of samples for presence of Vibrio can prevent outbreaks and health hazards. Detection of Vibrio species include enrichment in Alkaline Peptone water and further plating on selective media. The most commonly used selective medium includes TCBS Agar based on sucrose fermentation with bromothymol blue and thymol blue as indicator. Bile and high pH add to the selectivity of the medium. Other most common medium used for enumeration and isolation include selective and differential medium like CPC Agar base, Vibrio Agar, Proteose Agar, VP Medium, Vibrio Parahaemolyticus Sucrose Agar, Wagatsuma Agar Base. Chromogenic medium for Vibrio is more specific for Vibrio species wherein Vibrio cholerae gives purple colour, Vibrio parahaemolyticus gives green colour and Vibrio fluvialis gives blue colour. The medium is selective and inhibits the unwanted accompanying microflora.