HiPetriSlim™ S.aureus Count

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SKU:
HPS005
Recommended for the determination of total S.aureus in food, dairy and water samples.


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Intended use

Recommended for the determination of total S.aureus in food, dairy and water samples.

Composition

Proprietary

Staphylococcus aureus subsp. aureus

Formula adjusted, standardized to suit performance parameters

Directions

Open the pouch aseptically. Open the lid and add the required dilution of 0.5 ml to 1 ml. The solution will be evenly absorbed. Close the lid. Press the sides of the lid to ensure that it is fixed in the grooves. Allow 5-10 minutes for even absorption. Incubate the plates in horizontal position at specified temperature and period. After incubation, count the number of colonies which have appeared on the surface of the medium.

Principle And Interpretation

The medium is recommended for isolation and enumeration of Staphylococci in food and other material. The medium is highly nutritious and provides amino acids and long chain peptides for the growth of microorganisms. Staphylococci are capable of reducing tellurite, and the colonies appear as white to grey-black within 24-48 hours incubation at 35°C.

Salient features :

  • Recommended for testing liquid samples
  • Widespread Industrial application (Water, Food, Dairy & Cosmetics)
  • Compact packing reduces storage space
  • No Preparation time
  • User-friendly, ready-to-use products
  • Available in wide range of products that can be customized as per requirement

Type of specimen

Food and dairy industries, Cosmetic industries, Water and waste water testing laboratories, Other industries, laboratories where microbiological work is carried out.

Specimen Collection and Handling

Refer directions.

After use, contaminated materials should be autoclave at 121°C for 15 minutes before discarding.

Warning and Precautions

Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations

  1. Count Staphylococcus aureus like grey black colonies and test them for coagulase reaction.

Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control

Appearance
Sterile plastic plate containing cream to white nutrient pad

Sterility Check:
Passes release criteria.

Cultural Response:
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.

Organism Inoculum (CFU) Growth Colour of colony
Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) 50-100 luxuriant grey-black shiny
Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) 50-100 luxuriant grey-black shiny

Key: (*) - Corresponding WDCM numbers

Storage and Shelf Life

Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.

Disposal

User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).

More Information
Product Name HiPetriSlim™ S.aureus Count
SKU HPS005
Customized Product Available No
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