Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Ready Prepared Kits#4fa25a
Intended use
Recommended for the determination of Vibrio count in food and water samples by chromogenic method.
Composition
Proprietary
**Formula adjusted, standardized to suit performance parameters
Directions
Open the pouch aseptically. Open the lid and add the required dilution of 0.5 ml to 1 ml. The solution will be evenly absorbed. Close the lid. Press the sides of the lid to ensure that it is fixed in the grooves. Allow 5-10 minutes for even absorption. Incubate the plates in horizontal position at specified temperature and period. After incubation, count the number of colonies which have appeared on the surface of the medium.
Principle And Interpretation
Vibrio species are mainly responsible for causing cholera and food poisoning in humans. Vibrio cholerae causes cholerae due to the intake of contaminated food such as raw oysters. Vibrio parahaemolyticus is a major cause of food borne infections, causing food poisoning. The medium is highly nutritious and provides amino acids and long chain peptides for the growth of microorganisms. The enzyme beta-galactosidase helps in the chromogenic differentiation of Vibrio cholerae and Vibrio parahaemolyticus.
Salient features :
- Recommended for testing liquid samples
- Widespread Industrial application (Water, Food & Dairy)
- Compact packing reduces storage space
- No Preparation time
- User-friendly, ready-to-use products
- Available in wide range of products that can be customized as per requirement
Type of specimen
Food and dairy industries, Water and waste water testing laboratories, Other industries, laboratories where microbiological work is carried out.
Specimen Collection and Handling
Refer directions.
After use, contaminated materials should be autoclave at 121°C for 15 minutes before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Slight colour variation of Vibrio species may be observed depending upon strains, since it is an enzyme substrate based reaction.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the plate.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile plastic plate containing white to pale yellow nutrient pad
Sterility Check: Passes release criteria.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of Colony |
|---|---|---|---|
| Vibrio cholerae ATCC 15748 | 50-100 | good-luxuriant | purple |
| Vibrio parahaemolyticus ATCC 17802 (00037*) | 50-100 | good-luxuriant | bluish green |
| Escherichia coli ATCC 25922 (00013*) | >=104 | inhibited | |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: (*) - Corresponding WDCM numbers
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiPetriSlim™ HiCrome™ Vibrio Count |
|---|---|
| SKU | HPS010 |
| Customized Product Available | No |
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