Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Ready Prepared Kits#4fa25a
Intended use
Recommended for the determination of total E.coli in food, dairy and water samples by chromogenic method.
Composition
Proprietary
**Formula adjusted, standardized to suit performance parameters
Directions
Open the pouch aseptically. Open the lid and add the required dilution of 0.5 ml to 1 ml. The solution will be evenly absorbed. Close the lid. Press the sides of the lid to ensure that it is fixed in the grooves. Allow 5-10 minutes for even absorption. Incubate the plates in horizontal position at specified temperature and period. After incubation, count the number of colonies which have appeared on the surface of the medium.
Principle And Interpretation
HiPetriSlim™™ HiCrome™ E.coli Broth is used for detection and differentiation of Escherichia coli and coliforms in water samples. The medium is highly nutritious and provides amino acids and long chain peptides for the growth of microorganisms. The medium contains chromogenic mixture, which helps to detect glucuronidase activity of Escherichia coli (1). This specific enzyme differentiates Escherichia coli from other coliforms. Escherichia coli cells split the chromogenic mixture with the help of glucuronidase to give blue colouration to the colonies. Coliforms other than Escherichia coli turn red as they reduce TTC (2,3,5-triphenyl tetrazolium chloride).
Salient features :
- Recommended for testing liquid samples
- Widespread Industrial application (Water, Food, Dairy & Cosmetics)
- Compact packing reduces storage space
- No Preparation time
- User-friendly, ready-to-use products
- Available in wide range of products that can be customized as per requirement
Type of specimen
Food and dairy industries, Cosmetic industries, Water and waste water testing laboratories, Other industries, laboratories where microbiological work is carried out.
Specimen Collection and Handling
Refer directions.
After use, contaminated materials should be autoclaved at 121°C for 15 minutes before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Do not use diluents containing citrate, bisulfite or thiosulfate; they can inhibit growth.
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile plastic plate containing cream to white nutrient pad
Sterility Check:
Passes release criteria.
Cultural Response:
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) |
Growth | Colour of Colony |
|---|---|---|---|
| # Klebsiella aerogenes ATCC 13048 (00175*) | 50-100 | luxuriant | red |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | blue |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited |
Key: (*) Corresponding WDCM numbers, (#) Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiPetriSlim™ HiCrome™ E.coli Count |
|---|---|
| SKU | HPS004 |
| Customized Product Available | No |
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