Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Ready Prepared Kits#4fa25a
Intended use
Recommended for the determination of total E.coli and coliforms in water samples by chromogenic method.
Composition
Proprietary
**Formula adjusted, standardized to suit performance parameters
Directions
Open the pouch aseptically. Open the lid and add the required dilution of 0.5 ml to 1 ml. The solution will be evenly absorbed. Close the lid. Press the sides of the lid to ensure that it is fixed in the grooves. Allow 5-10 minutes for even absorption. Incubate the plates in horizontal position at specified temperature and period. After incubation, count the number of colonies which have appeared on the surface of the medium.
Principle And Interpretation
HiPetriSlim™™ HiCromeтм E.coli-coliform Water Count is recommended for the simultaneous detection of Escherichia coli and total coliforms in water. The medium is highly nutritious and provides amino acids and long chain peptides for the growth of microorganisms. The enzyme ß-glucuronidase produced by E.coli utilizes the chromogenic substrate to produce blue-purple coloured colonies. Coliforms other than Escherichia coli turn red as they reduce indicator dye. Thus, the resulting colour distinction allows simple interpretation of test without further confirmation.
Salient features :
- Recommended for testing liquid samples
- Widespread Industrial application (Water, Food, Dairy & Cosmetics)
- Compact packing reduces storage space
- No Preparation time
- User-friendly, ready-to-use products
- Available in wide range of products that can be customized as per requirement
Type of specimen
Water and waste water testing laboratories, Other industries, laboratories where microbiological work is carried out.
Specimen Collection and Handling
Refer directions.
After use, contaminated materials should be autoclave at 121°C for 15 minutes before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Do not use diluents containing citrate, bisulfite or thiosulfate; they can inhibit growth.
- B-glucuronidase is present in 97% of E.coli strains, however few E.coli may be negative.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Sterile plastic plate containing pale blue to blue nutrient pad.
Sterility Check
Passes release criteria.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Colour of Colony |
|---|---|---|---|
| Citrobacter freundii ATCC 8090 | 50-100 | luxuriant | red |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant | blue |
| Escherichia coli ATCC 35218 | 50-100 | luxuriant | blue |
| Enterococcus faecalis ATCC 29212 (00087*) | >=104 | inhibited | - |
| Klebsiella pneumoniae ATCC 13883 (00097*) | 50-100 | luxuriant | red |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | >=104 | inhibited | - |
| Staphylococcus aureus subsp. aureus ATCC 6538 (00032*) | >=104 | inhibited | - |
Key: (*) Corresponding WDCM numbers
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
| Product Name | HiPetriSlim™ HiCrome™ E.coli-coliform Water Count |
|---|---|
| SKU | HPS007 |
| Customized Product Available | No |
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